Purpose: Restriction enzymes cut DNA at a certain palindromic sequence. Three samples of lamda DNA set up to be cut with restriction enzymes PstI‚ EcoRI‚ or HindDIII. There were also two more samples‚ one of these samples was not mixed with any restriction enzyme and the other was a marker‚ which used an enzyme which creates fragments with a known number of base pairs used to create a standard curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of
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Enzymes are biological catalysts and each individual enzyme can only catalyse to one type of reaction – due to its specific shape. Each individual enzyme has its own specific shape which is determined by the amino acid sequence that it is made up of – each enzyme’s active site matches to its unique substrate molecule. For the sake of our experiment – enzymes catalyse reactions because they become an active site for reactions to take place. This lowers the energy that is needed for the reaction but
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pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution
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find the concentration (mole/dm3 (M)) of solute in a potato cell by using the process of osmosis and different concentrations of sucrose solution. Background information Osmosis is diffusion of water across a partially permeable membrane. It moves from a solution with less solute concentration (high water potential) to a solution with more solute concentration (low water potential). The one with a high water concentration is called a hypotonic solution and the low water concentration is called
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Discussion: Shown in tables 1 and 2‚ the shoot heights‚ on average‚ of the barley seeds‚ increased as the Aquasol concentration increased. This is with the exception of the solution with an initial Aquasol concentration of 0.40% in Table 1‚ which showed to have‚ on average‚ shorter shoot heights in comparison to the solution with 0.25% Aquasol. Ultimately‚ this indicates that the experiment had random and systematic errors that affected the accuracy‚ reliability‚ validity‚ and precision of the results
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Event When World War II began there were 9 million people in concentration camps‚ when the war ended there were 3 million people. When the Japanese attacked Pearl Harbor President Ford put the japanese americans and the immigrants into internment camps because they looked like the enemy‚ the Japanese were not able to fight in the Military because they looked like the enemy. When Hitler became Chancellor he chose to put the Jews in concentration camps because he thought they looked like the enemy‚ Hitler
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million Jews” (Fact Retriever 1). Concentration camps were responsible for this act of mass murder. Many would hide for from those responsible for the camps. The Nazis built and run these camps to imprison those accused of committing crimes against the state or known as “enemies of the state”. During the holocaust‚ concentration camps left a mark on our society. First‚ they were created to detain so-called “enemies of the state”‚ so they were named concentration camps. In addition‚ the Nazi’s had
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Part B: Practical Report The Effect of Temperature on Enzyme Activity Aim: To investigate how temperature effects the enzyme catalase. Hypothesis: If the temperature of water is increased then the enzyme will react quicker to form oxygen and water‚ when compared to cold water. Purpose: To design and conduct a plan of a practical about the effects of temperature on enzymatic activity with a partner. Introduction: An enzyme is a protein‚ which speeds up a specific chemical reaction without altering
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Running head: LAB 5: INVESTIGATING AN ENZYME-CATALYZED REACTION Lab 5: Investigating an Enzyme-Catalyzed Reaction September 24‚ 2014 Principles of Biology 120.601 Mrs. Annemarie Duncan Abstract: (Burmania) This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in
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The definition of holocaust is death by fire. Concentration camps were built to kill the inferior people. To Hitler‚ Concentration Camps were part of his finale solution of exterminating the inferior people to make his master race. In 1939‚ the Nazis had defeated Poland. Poland had around of 3‚000‚000 Jews. The Concentration Camps were located all over both East Germany and Poland. As the war went on and the Nazis kept moving forward they had to build more camps. The camps were spread out all over
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