The Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of
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Retrieved October 29‚ 2013‚ from http://prezi.com/wii0fgw48euc/potatoe-enzyme-lab/ What enzyme is present in liver and potatoes. (n.d.). Retrieved October 29‚ 2013‚ from http://wiki.answers.com/Q/What_enzyme_is_present_in_liver_and_potatoes#slide2 What Is the Optimum PH for Catalase - Ask.com. (n.d.). Retrieved October 29‚ 2013‚ from http://www.ask.com/question/what-is-the-optimum-ph-for-catalase What is the optimum PH for potato catalase and why? - Yahoo! Answers. (n.d.). Retrieved October 29‚ 2013
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concentration. Hypotonic solution is the solution with the lower salt concentration. My hypothesis is that the potato core will increase in size. When the solution is hypertonic‚ the potato will decrease in size making the potato hypotonic. Osmosis is really interesting‚ that is why I chose to write this report about it. Materials and Methods We first cut out six cores out of a potato. We trimmed them all to the same length using a razor. After we had cut them to the same size we weighed them using
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3%‚ was diluted using 10 mL of hydrogen peroxide and 20 mL of water. In the second beaker‚ a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay was performed. Using forceps a small paper disk was dipped into the second beaker containing the potato catalase for exactly 5 seconds. It was next dried on a paper towel for 5 seconds‚ and finally placed at the
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which came from the potato juice. The function of an enzyme is to catalyze reactions in cells. The specific function of the enzyme in this experiment was to see if it affected the activity if it was exposed to different temperatures. A substrate is a material or substance which the enzyme acts. The substrate in our lab was hydrogen peroxide. Procedure For our experiment we used‚ three test tubes‚ nine milliliters of potato
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Fermentation of a Carbohydrate: Ethanol from Sucrose Abstract The purpose of this lab was to demonstrate the fermentation process of ethanol from the substrate sucrose. To make ethanol from sucrose two enzymes invertase and zymase were used. Vacuum filtration and fractional distillation were performed to get a more concentrated solution. The density of ethanol was .825 g/mL with a percent composition of 85% pure
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Title: Potato Osmosis Abstract: In this experiment was designed to study the effect of a concentrated solution applied to white and sweet potatoes. The solution’s impact among the potatoes were to be either hypertonic‚ hypotonic‚ or isotonic. The potatoes were first cut into fries with a fry cutter‚ and then weighed after being cut. The white and sweet potatoes’ weights ranged from .005-.015 grams. A total of 18 sugar solutions are to be made over the course of 3 trials. In each trial‚ 6 different
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Estimating Osmolarity of Plant Cells Criteria Assessed: DCP Introduction In this experiment you will be estimating the osmolarity of potato cells by finding their change in weight in solutions of known molarity. The object is to find the molarity where the mass does not change. No net change in weight indicates that there has been no net gain or loss of water. This is a means of indirectly finding the osmolarity of the cells themselves. Apparatus 1 large potato Petri dish 7 x 250 ml beakers
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experiment‚ you will study osmosis. Your task is to design an experiment that will determine what concentration of solute is isotonic with the specimen cells. (Design) Procedure: You will be provided with the following materials: Potato‚ potato corer‚ scalpel‚ forceps‚ electronic balance 10 % salt solution‚ distilled water‚ 50 mL beakers‚ graduated cylinder‚ paper towel You must design a procedure that will enable you to determine the concentration of salt that is isotonic
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Keegan Bauman Potato Lab Dr. Peano Block 1 Introduction: Potatoes are just your average food; they have been around for as long as the human race can remember. In this lab we will make them be a prime example of osmosis‚ which is a transport mechanism. In this lab the problem being tested was what environment affects the mass of the potatoes the most. The independent variable is the environments used salt‚ water and iodine solution. The dependent variable is the mass of the potato after being
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