The purpose of the laboratory investigation was to observe the diffusion of water across a semi-permeable membrane. In this lab‚ dialysis tubing was used to represent the semi-permeable cell membrane. Three dialysis tubes were prepared with 5% sucrose solution and were soaked in solutions of different concentrations. The diffusion of water across a semi-permeable membrane is called osmosis. When water diffuses‚ the water molecules move from a hypotonic environment to a hypertonic environment
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unknown sucrose solutions. First I would pour an equal volume of each solution into beakers labeled A‚ B‚ C‚ and D. I would also have a beaker of the same amount of distilled water to serve as a control for the experiment. Then I would obtain several baby carrots‚ 4 per beaker. I would mass the groups of 4 potatoes before placing them in their respective beakers of solution. potatoes do have semipermeable cell membranes (water should be able to pass‚ but not necessarily the larger sucrose)‚ but in
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Osmosis and Scientific Method Lab Report Abstract The purpose of the lab is to see how different size potatoes have faster water movement depending on the concentration of sucrose it was placed in. We had two sized potatoes‚ 6g and 10g‚ and placed each in three different dilutions of sucrose at 0.5M‚ 0.1M‚ and 0.05M. We also placed a 6g and 10g potato piece in water to act as a control. Based on the weight percent change over 60 minutes‚ the 6g potato piece had faster water movement than the
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we studied the nature of enzyme actions using live yeast cells as our source of sucrose. The enzyme will then break the sucrose into one molecule of glucose and fructose. Because sucrose is a large molecule that cannot enter most cells‚ yeast will produce sucrase and secrete it into cell membranes. The sucrose will be hydrolyzed into small six-carbon monosaccharide’s which can enter into the cell membranes. The sucrose can be obtained from a 0.5 percent solution of “dry baker’s yeast in water”.
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Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity. 1. Dependent Variable. amount of product
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different concentrations of sucrose Alex McRae Biology 120-902 Grand Valley State University 1 Campus Drive Allendale‚ MI 49401 mcraeal@mail.gvsu.edu Abstract In this study‚ we tested the validity of osmosis in artificial animal cells. Osmosis is the diffusion of free water across a membrane. The purpose of the study was to calculate the rate of osmosis in artificial cells containing different concentrations of sucrose and water. We studied the
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securely so that the solution would not spill. Approximately 15mL of 0.4 sucrose solution was poured into one tube‚ while 15mL of 0.6 sucrose solution was poured into the other. Using our fingers‚ we removed most of the air out of the tube‚ and tied off the other end of the bag with string. Sufficient space was left so that the contents could expand. Both bags were then rinsed off gently with distilled water to remove any sucrose solution spilled. Then the outside of the bags were blotted off and the
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investigate the effect of sucrose concentration on the rate of cellular respiration in yeast. Under specific conditions‚ yeast will convert sucrose into glucose and then use this glucose in cellular respiration. Yeasts have been used by humans in the development of civilization for millennia. What is yeast? How have humans used yeasts? * Yeast is a fungi‚ it grows. Humans use much yeast in our bread today‚ because it makes our bread rise. * What is sucrose? How would sucrose be used as food source
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Diffusion Experiment: Osmosis in Sucrose Solutions Abstract We used dialysis tubing to simulate a semi-permeable membrane. Since molecules diffuse from their higher concentration to their lower concentration‚ water will move across the membrane in response to this concentration. While conducting this lab we were able to observe passive transport through diffusion and osmosis. Introduction Osmosis is a specialized case of diffusion that involves the passive transport of water. When
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Direction and concentration gradients Bradley Benton ABSTRACT In this experiment‚ we will investigate the effect of solute concentration on osmosis. A semi‐permeable membrane (dialysis tubing) and sucrose will create an osmotic environment similar to that of a cell. Using different concentrations of sucrose (which is unable to cross the membrane) will allow us to examine the net movement of water across the membrane. INTRODUCTION A major determinant of diffusion in a biological system is membrane
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