2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity. 1. Dependent Variable. amount of product (glucose
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Abstract: Water potential is the measure of free energy of water in a solution. The purpose of this lab was to find the water potential of a potato cell. The problem of this lab was‚ if there was a high molarity of solute‚ would it have a positive or negative water potential? The hypothesis of the experiment was‚ if there is a high molarity of solute then the water potential would be lower or negative. After obtaining potatoes‚ we cut them into pieces‚ weighed them‚ and then placed them in a solution
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setup area. 2. After gently blotting‚ weigh each egg separately to the nearest 0.1g and record the weights in Table 1 at time “0”. 3. Place eggs 1‚ 2‚ and 3 into separate culture dishes containing solutions of distilled water 0% sucrose‚ 14% sucrose‚ and 40% sucrose respectively. 4. At 15 minutes intervals‚ that is after 15‚ 30‚ 45‚ 60‚ and 75 minutes‚ remove the eggs from the dishes; carefully wipe off all excess water‚ and again weigh each egg separately. Record the weight in Table 1. 5. Replace
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concentration of molecules are identical inside and outside. Also‚ we predicted that if the carrot sticks were placed into higher concentrations of sucrose solutions‚ then the sticks would loose more water. The concentration of particles inside the carrot cells will be found by having them placed into various different solutions with different sucrose concentrations. This will cause the carrot cells to either lose or gain water as the control tries to reach equilibrium with the solution. By measuring
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higher the concentration of solute‚ the higher the mass of the solution. Methods and Materials: For part A of the experiment‚ the following items are needed: Dialysis tubing‚ IKI solution‚ 15% glucose/1% starch solution‚ distilled water‚ balance‚ sucrose solutions (0.2M‚ 0.4M‚ 0.6M‚ 0.8M‚ 1.0M)‚ glucose test tape‚ beakers (multiple)‚ petri dish. In the experiment‚ one will measure diffusion through a selectively permeable membrane (dialysis tubing). A 30 cm by 2.5 cm piece of dialysis tubing that
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The sucrose will test negative due to the fact that it is a carbohydrate and not a composition of organic material but rather a single part. In the test for starches‚ water again will serve a the negative control for the experiment becasue it is inorganic. it appears obvious the 1% starch solution will test positve for starches becasue it is starch. The onion juice should carry starch due to the fact that starch is stored energy for plants and onions are very bulbous‚ while the sucrose solution
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a microscope when it was placed in different solutions. The first onion membrane I observed was placed in water. The cells looked swollen and tightly compacted. When I observed the onion skin in 0.3M sucrose‚ the cells were loosely compacted. I last observed the onion membrane in 2.0M sucrose and the cells looked very thin and far apart from each other in comparison to the other slides. Table 1 shows the data I
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sweetener alternative to sucrose (common table sugar) first introduced to the food and beverage industry in the 1970s. It is not meaningfully different in composition or metabolism from other fructose-glucose sweeteners like sucrose‚ honey‚ and fruit juice concentrates. HFCS was widely embraced by food formulators‚ and its use grew between the mid-1970s and mid-1990s‚ principally as a replacement for sucrose. This was primarily because of its sweetness comparable with that of sucrose‚ improved stability
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Their preference for these sweeteners vary depending on their concentration. To understand the specific concentration of sucrose preferred by Drosophila‚ we exposed them to a serial dilution of sucrose. After obtaining the concentration of sucrose that is most preferred‚ we created a serial dilution of quinine with this concentration to examine whether Drosophila could detect different concentrations of quinine. Since aging in humans
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tissue in sucrose solutions of different concentrations and measuring the changes in mass of the apple cores. The changes in mass reflect the movement of water into or out of the tissue. After determining the concentration of sucrose solution at which there is no net gain or loss of water from the apple tissue‚ the osmotic potential can be calculated. This will then tell the water potential of the apple cells. Research Question and Hypothesis: How will changing the concentration of sucrose in solutions
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