Objective: DNA is analyzed by agarose gel electrophoresis after being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then‚ EcoRI buffer‚ EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used to bring
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on on studying DNA by using a technique called X-ray diffraction. Her expertise was the X-ray diffraction mostly of proteins and lipids in a solution on DNA fibers. Franklin and a student‚ Raymond Gosling‚ took pictures of DNA and made the observation that there are two forms of DNA; the “A” form‚ which is dry and the”B” form‚ which is wet. One of their pictures of this observations‚ known as Photograph 51‚ became famous and was analytical evidence in discovering the structure of DNA. the photograph
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Product Manager‚ Phoebe Masters‚ at Ms.-Tique Corporation must decide if and how the company will introduce an aerosol can package for its Soft and Silky Shaving Gel‚ including a determination of product size (5.5 or 10 oz.) and location (personal care or toiletries). Furthermore‚ the manager needs to decide if a test market is necessary to evaluate the new packaging. Strategic Issues Product Soft and Silky Shaving Gel (SSSG)‚ a women’s shaving product that contains moisturizers‚ is currently
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MAT 111 1) Tells the story of your mathematical history. How would you describe what mathematics is in your own words? What is mathematics to me? Mathematics is the study of numbers and also concepts and shapes. Math can be a lot of thing in math it can include numbers‚ letters are shape and lines .Do you consider yourself good at mathematics? No I don’t. Why; I don’t think I’m good in math is because I’m not patience enough to solve the problem in math. Was there a time in your life
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plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal DNA from your
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DNA Lab Report SungYong Jang What is DNA? What do the letters stand for? What is it composed of? Where is it found? What is it shaped like? Answer in full sentences. It is the genetic material that can be passed on from parent to offspring DNA --> Deoxyribonucleic acid It composed of Deoxyribose (5 carbon sugar)‚ a phosphate and a Nitrogonous base (the 4 N-bases are Guanine‚ Cytosine‚ Adenine and Thymine) It is a double helix (looks like
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- Summarize the viewpoints given in the option. In other words‚ what are the ins and outs of the option? This ideology believed that abolitionism did not have to be violent and could be quickly as long as the slave owners were requited. This would include a sort of “buying” the slaves and then letting them free. This would leave the old slave owners compensated and would allow the slaves to go free. Along with no violence this is an ideal solution. Although this idea sounds perfect‚ there were
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and gel. We will be adding different substances such as table salt‚ citric acid‚ and sugar. The scientist’s problem statement is “What kind of substances make gel the strongest?” The independent variable will be the different additives (Table salt‚ citric acid‚ and sugar) which will be added using tablespoons. The dependent variable is the strength of the gel which will be measured using a scale of 10. The constant variable is the type of gel used and the amount of substance added to the gel which
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Question 1 1.1 Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the
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that is extremely flammable. It is made from a mixture of a gel and gasoline. Napalm has been used in World War 2‚ but became famous in the Vietnam War. It was first used as a weapon against buildings‚ but later it became a weapon against other soldiers. Napalm was very effective against people‚ because it sticks to the skin and causes extreme burn wounds. Napalm is quite an inhumane weapon. When napalm was created in World War 2‚ it was used in flamethrowers. Later in World War 2‚ Napalm was put into
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