Materials and Methods Growing the G Strain and Preparing the GCE (rGFP Crude Extract): To grow the bacterial culture‚ use 10 ml of liquid LB growth media for incubation. 500 ml of the bacterial culture is allowed to grow overnight at 37°C. It is later shaken vigorously to increase the OD600 to 0.5‚ which means that time equals zero. At time zero‚ 1 mL of the culture is transferred into a 1.5 mL centrifuge tube and centrifuged for 5-10 minutes to obtain a pellet. The supernatant should be discarded
Premium Western blot Molecular biology Gel electrophoresis
May 29th‚ 2013 Course code: 7308 Dr. Sharon Avital Student: 000776790 Theories of Persuasion Writing Assignment Hello‚ my name is Santos Serfaty and I am a recruiting officer representing the LOST (Learn on Saturdays too) Collective‚ and together we are devoted to restructure the undergraduate curriculum so that the students must attend six days a week‚ between 4 to 6 hours a day to the IDC. As a current student at the IDC school of Communications I strongly believe that memory is set
Free High school College Education
Cheyenne Pruett Ms. Mitchell College Composition October 8‚ 2013 Ophelia? Thomas G. Plummer‚ in his essay “Diagnosing and Treating the Ophelia Syndrome” argues that the system we use for teaching today takes away students ability to think for themselves. Plummer proves his argument with loads of examples of students conforming to others to take the easy way out. In Plummers essay he points out ways to treat this syndrome and stop all ignorant people from ruining the minds of other
Premium Carl Jung Emotion Writing
Music History Review: Romantic The Norwegian composer Edvard Grieg penned in a letter that he wrote to his friend Gottfred Mattison-Hansen on July 30‚ 1867‚ his second violin sonata “in the euphoria of my honeymoon”.1 This sonata in G major was a labour of affection that the composer wrote in honour of the love of his life Nina Hagerup‚ who was his cousin‚ whom he married despite familial disapproval on both sides. The evident happiness is reflected in the demeanour and beauty of the work
Premium Edvard Grieg Norway Violin
moving forward. The combination of technological devices with educations provide further interests and purposes for students to learn more knowledge and information that traditional educational institutions do not approach and access. Furthermore‚ Susan Faludi‚ author of "The Naked Citadel‚" discusses how the institution in a military college‚ The Citadel‚ regulates every male student to develop mutual understandings and perceptions that can hardly avoid and ignore. The system inside the school distinct
Premium Personal life Psychology Economics terminology
Topic: S.992 Persuasive Speech of Public Policy I. Purpose of presentation A. General Purpose: To persuade B. Specific Purpose: To persuade the audience to take action to urge their congress person to vote against S.992 - The National Nursing Shortage Reform and Patient Advocacy Act II Introduction / Attention A. Gain attention: Picture this. Your loved one had surgery yesterday. A nurse is fixing their morphine drip when their manager comes in and tells the nurse they are required to take
Premium Nurse Nursing Patient
Inventory Optimization at Procter & Gamble: Achieving Real Benefits through User Adoption of Inventory Tools Procter & gamble (P&G) is a leading global consumer products company founded in 1837 with $76.7 billion in sales in 180 countries. P&G is famous for their brand names like Tide‚ Gillette‚ Pantene and many others. It competes in 26 distinct product-category markets such as hair care‚ cosmetics‚ paper towels‚ skin care‚ oral care‚ blades and razors‚ diapers and fabric care. Procter &
Premium Supply chain management Inventory Supply chain
Metropolitan‚ Micropolitan‚ and Rural Communities by Jennifer Cole and Ginny Sprang‚ Challenges to Identifying and Prosecuting Sex Trafficking Cases in the Midwest United States by Andrea J. Nichols and Erin C. Heil‚ and Prostitution on Demand by Janice G. Raymond each have their own unique perspective on the topic of sex trafficking. The articles sample from and did their research in different areas of interest in the topic of sex trafficking‚ which is why they are all inimitable. Sex Trafficking of
Premium Human trafficking Prostitution
Melissa Prather English 102 Research Paper May 8th‚ 2012 Understanding Feminism in Susan Glaspell’s Trifles Susan Glaspell lived during a time where women’s rights were not fully acknowledged. The oppression of women during this time stretched to the point that they were not truly acknowledged as their own person. They were to be seen and not heard so to speak. Their sole purpose was to take care of their families by keeping house and performing their caretaker duties. Glaspell even demonstrates
Premium Susan Glaspell Woman Wife
Associate Level Material Appendix G Security Assessment Directions Choose two settings from Ch. 9 of the text and list each setting. Then‚ complete the following tables. List five threats appropriate to the environment from the setting you chose. Rate the risk for each threat from 0 (low) to 10 (high). Then‚ list five appropriate countermeasures‚ including at least two countermeasures specific to physical security and one countermeasure specific to personnel and security management.
Premium Computer security Physical security Security