The main goal of this experiment was to successfully clone human insulin cDNA into an expression vector. The gel electrophoresis results confirm that the inserts were successfully cloned into the expression vector based on the identical fragment position of each cut candidate in the gel. The fragment sizes of the both cut candidates produced on the gel when compared to the DNA ladder were larger‚ at roughly about 6000 base pairs and 6800 base pairs‚ than the predicted size of 2315 and 3119 base pairs
Premium DNA Genetics Gene
Secondary excitotoxicity is thought to be more relevant to chronic neurodegenerative disorders than the classical acute form. In this pathway increased glutamate levels are not required as disturbances with the neuronal resting membrane potential cause glutamate receptors to be over-activated by physiological levels of glutamate. In a neuron which is affected by another pathological process and shows reduced ATP production‚ Na+ dysfunction or impaired glucose metabolism and as a result has an abnormal
Premium Neuron Nervous system Brain
The movie Juno is fiction and came out in 2007. It is about Juno MacGuff a 16 year old high schooler who gets pregnant after her first time having intercourse with Paulie Bleeker‚ who is also in high school. Although 9 years old‚ this movie is still extremely relevant in today’s society in regards to teen pregnancy. It tackles all the different options one can take and how emotionally‚ physically‚ and mentally challenge this decision is‚ especially when you’re a teen. Psychologist David Elkind theorized
Premium Family Pregnancy Mother
The nonmutated form of DMPK functions in the production of protein kinase serine; this enzyme plays a role in intracellular communication and in the regulation of myosin phosphate. When DMPK is mutated‚ the proteins CUG-BP1‚ MBNL1 and INSR are displaced causing the splicing of troponin pre-mRNA which causes malfunctions in the cardiac conduction system‚ dominance of a chloride channel that causes myotonia‚ dominance of an insulin receptor that affects the sufficiency of insulin‚ and dominance of
Premium Protein DNA Gene
Introduction: The pharmacokinetics of a drug describes the journey of a drug through the body i.e. its absorption‚ bioavailability‚ distribution‚ metabolism and excretion. Opioids are substances (endogenous or synthetic)‚ which produce ‘opiate-like effects’. Opiates are alkaloids derived from the Papaver somniferum poppy e.g. Morphine‚ Codeine (a morphine-like agonist) and Methadone (a synthetic analogue of morphine) and are primarily used as analgesics.[1] Discussion: Morphine has a 4-ring structure
Premium Opioid Morphine Drug addiction
contains four sections‚ SECTION A to SECTION D. Instructions: Answer all questions in SECTION A - SECTION D. Make sure that the section heading is included and your answers are correctly numbered. The assignment must have a completed cover sheet. It must be placed in the drop-box on or before the deadline. st SECTION A ELECTRONIC STRUCTURE & IONIZATION ENERGY 1. 2. Write the electronic structure in s‚p‚d notation of the following: O‚ Na‚ Na+‚ Al‚ Cl- and Co
Premium Sodium hydroxide Enthalpy Hydrochloric acid
November 15‚ 2007 Synthesis of 1-Bromobutane Introduction: The purpose of this experiment is to synthesize 1-bromobutane from 1-butanol and sodium bromide. In order for this reaction to reach completion there are four major operations that need to be performed. The four major operations include refluxing‚ simple distillation‚ separation‚ and drying. To begin‚ in order for the compounds to react they will be dissolved in water and sulfuric acid will be added. The addition of sulfuric acid will
Premium Distillation
purpose of a synthesis essay is to make insightful connections. Those connections can show the relationship(s) between parts of a work or even between two or more works. It is your job to explain why those relationships are important. In order to write a successful synthesis essay‚ you must gather research on your chosen topic‚ discover meaningful connections throughout your research‚ and develop a unique and interesting argument or perspective. A synthesis is not a summary. A synthesis is an opportunity
Premium Writing Essay Logic
9.3 – The Acidic Environment: Δ. Construct word and balanced formulae equations of all chemical reactions as they are encountered in this module: NOTE: In chemistry‚ [x] means “concentration of x” in moles per litre (mol/L). EG: [H3O+] means “concentration of H3O+ ions” in mol/L. BASIC reactions to remember: Acid reactions: acid + base salt + water acid + metal salt + hydrogen gas acid + carbonate carbon dioxide gas + salt + water Formation of hydronium: H+ + H2O H3O+ Reactions of
Premium Acid PH
Introduction The American Chemical Society databases were investigated through Scifinder Scholar Version X to find any information about Butyl 2-Cyano-(3‚5 dichloro-phenyl)-2-propenoate. There was no previous information present. Then‚ the microscale synthesis and characterization of Butyl 2-Cyano-(3‚5 dichloro-phenyl)-2-propenoate was conducted with the base catalyzed Knoevengael condensation of the butyl cyanoacetate and corresponding aldehyde (gkharas/235_14F/235_14F_Lab_2.pdf) and analysis. The elemental
Premium Thin layer chromatography Stoichiometry 3rd millennium