The main goal of this experiment was to successfully clone human insulin cDNA into an expression vector. The gel electrophoresis results confirm that the inserts were successfully cloned into the expression vector based on the identical fragment position of each cut candidate in the gel. The fragment sizes of the both cut candidates produced on the gel when compared to the DNA ladder were larger‚ at roughly about 6000 base pairs and 6800 base pairs‚ than the predicted size of 2315 and 3119 base pairs
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The movie Juno is fiction and came out in 2007. It is about Juno MacGuff a 16 year old high schooler who gets pregnant after her first time having intercourse with Paulie Bleeker‚ who is also in high school. Although 9 years old‚ this movie is still extremely relevant in today’s society in regards to teen pregnancy. It tackles all the different options one can take and how emotionally‚ physically‚ and mentally challenge this decision is‚ especially when you’re a teen. Psychologist David Elkind theorized
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A co-op store is a store which is owned and controlled by members of a cooperative‚ rather than a single store-owner or corporate entity. The cooperative tradition dates back to the 1700s in Great Britain‚ when workers began organizing in cooperatives in an attempt to get around usurious prices for basic goods‚ housing‚ and other services. Today‚ cooperative stores can be found all over the world‚ and some of these stores are extremely successful business entities. The idea behind a co-op store
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Chapter 3 Case: The Dim Lighting Co. Arshini Dhambarage Baker College Chapter 3 Case: The Dim Lighting Co. Name: Jim West 1. Problems A. Micro Short sightedness of the Accounting and Manufacturing departments. Competitor threats. B. Macro Profit margin dropped by 15% (poor financial situation) Company is not in a position to spend the capital necessary to fund the project. The potential resignation of Robert Spinks if the project is not funded. If the project
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ACT OF 1934 For the fiscal year ended December 31‚ 2013 or ¨ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from ________ to ________ Commission File No. 1-7259 Southwest Airlines Co. (Exact name of registrant as specified in its charter) TEXAS 74-1563240 (State or other jurisdiction of (IRS Employer incorporation or organization) Identification No.) P.O. Box 36611 Dallas‚ Texas 75235-1611 (Address of principal executive
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Islam have to say regarding co-education in schools? What are your viewpoints regarding this? Please elaborate on the pros and cons of such a system. Two popular arguments in favor of co-education are: 1. Coeducation builds confidence in a person and makes him/her a more complete person to live in a real world. In other words‚ studying in the same-sex education system makes a person lack in self confidence. 2. Another argument given by the proponents of co-education is that a person’s
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hierarchical layer Computer Organization – Module 1 Two different figures showing the organization of a computer‚ showing the five classic components pa ge -An abstraction omits unneeded helps us cope with complexity detail‚ s. co 0.1 What is happening below Your Program? m 1 Introduction & MIPS http://handoutsuite.googlepages.com Little bit of History “Where . . . the ENIAC is equipped with 18‚000 vacuum tubes and weighs 30 tons‚ computers in the future may
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SYNTHESIS OF AN ALKYL HALIDE S.M. BLANCAFLOR1 1INSTITUTE OF BIOLOGY‚ COLLEGE OF SCIENCE UNIVERSITY OF THE PHILIPPINES‚ DILIMAN‚ QUEZON CITY 1101‚ PHILIPPINES DATE SUBMITTED: 3 JANUARY 2012 DATE PERFORMED: 8 DECEMBER 2011 ------------------------------------------------- ABSTRACT Alkyl halides are molecules which have a carbon atom attached to a halogen atom (e.g.‚ chlorine‚ iodine‚ or bromine.) Alkyl halides are very important since they are used in many of the products used today
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October 9‚ 2013 Partner(s): Acetylsalicylic Acid Synthesis Theory. This experiment was carried out to see how the hydroxyl group on the benzene ring in salicylic acid reacts with acetic anhydride to form an ester‚ and to make aspirin. Synthesis of Acetylsalicylic Acid occurs by protonation of carbonyl (C=O) group‚ and a nucleophilic attack of OH on the acetic anhydride. The ferric chloride test and melting point were used to test the purity of the results. A hypothesized recovery rater of above
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was distilled to yield a pure tert-butyl chloride. The tert-butyl alcohol‚ together with HCl‚ underwent an SN1 nucleophilic substitution reaction‚ which is composed of three steps—(1) propanation of alcoholic oxygen‚ (2) formation of carbocation‚ and (3) attacking of the nucleophile to the carbocation. The experiment was only able to yield 0.504 grams or 5.21% of the theoretical yield of 9.679 grams‚ which is considered as extremely low percent yield. This result could have resulted from various experiment
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