The main goal of this experiment was to successfully clone human insulin cDNA into an expression vector. The gel electrophoresis results confirm that the inserts were successfully cloned into the expression vector based on the identical fragment position of each cut candidate in the gel. The fragment sizes of the both cut candidates produced on the gel when compared to the DNA ladder were larger‚ at roughly about 6000 base pairs and 6800 base pairs‚ than the predicted size of 2315 and 3119 base pairs
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Secondary excitotoxicity is thought to be more relevant to chronic neurodegenerative disorders than the classical acute form. In this pathway increased glutamate levels are not required as disturbances with the neuronal resting membrane potential cause glutamate receptors to be over-activated by physiological levels of glutamate. In a neuron which is affected by another pathological process and shows reduced ATP production‚ Na+ dysfunction or impaired glucose metabolism and as a result has an abnormal
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The nonmutated form of DMPK functions in the production of protein kinase serine; this enzyme plays a role in intracellular communication and in the regulation of myosin phosphate. When DMPK is mutated‚ the proteins CUG-BP1‚ MBNL1 and INSR are displaced causing the splicing of troponin pre-mRNA which causes malfunctions in the cardiac conduction system‚ dominance of a chloride channel that causes myotonia‚ dominance of an insulin receptor that affects the sufficiency of insulin‚ and dominance of
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Introduction: The pharmacokinetics of a drug describes the journey of a drug through the body i.e. its absorption‚ bioavailability‚ distribution‚ metabolism and excretion. Opioids are substances (endogenous or synthetic)‚ which produce ‘opiate-like effects’. Opiates are alkaloids derived from the Papaver somniferum poppy e.g. Morphine‚ Codeine (a morphine-like agonist) and Methadone (a synthetic analogue of morphine) and are primarily used as analgesics.[1] Discussion: Morphine has a 4-ring structure
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USPS Synthesis Essay If I were the current CEO for the United States Postal Service‚ I would be gravely concerned about the future of my business‚ as it has recently taken a steep plummet from its success and popularity in the 19th and 20th centuries. The USPS is now faced with the decision to renovate itself due to the extreme loss of business as technology takes over the 21st Century. Some say that the USPS should be entirely reconstructed because the business is only headed further downhill
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Nikki Vietz Ms Bontekoe Honors English 11 3 October 2013 Synthesis Essay By changing the word “nigger” to “slave” defeats the purpose of the Adventures of Huckleberry Finn being a historical piece of literature. Such a change means ignoring the past and the word “nigger” is part of that past. “Nigger” is demeaning and insulting but is its replacement any less demeaning and insulting. Changing the word would not only destroy the message that Mark Twain was trying to convey‚ but also America’s history
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Hate speech as defined by the legal dictionary is described as follows: “Hate speech is spoken words that are offensive‚ insulting‚ and/or threatening to an individual or group based on a particular attribute of that person or persons being targeted. Targeted attributes include such traits as ethnic background‚ sexual orientation‚ race‚ or disability‚ though there are other target attributes. In the U.S.‚ another term for hate speech is “fighting words‚” as such talk is likely to provoke an
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In Amy Tan’s The Joy Luck Club‚ from “Two Kinds” and in Bissinger’s Friday Night Lights‚ the outcome of parents expectations of their children is perfectly portrayed. When a child is pressured to live up to their parents expectations‚ they become stressed. In most cases‚ this leads to failure because the child feels as if they will never be able to please the parent. In other cases it leads to the child cracking under pressure and being too anxious to complete the task at hand‚ or the child might
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Look around you‚ what do you see? Violence‚ it’s everywhere on the news‚ in articles‚ even in our conversations. People spread acts of violence through talking and writing. But without that act of violence in the beginning there wouldn’t be a story. “Through violence you may ‘Solve one problem’ but you sow the seeds for another‚” -Dalai Lama. Acts of violence are like falling dominos‚ one act leads to another just like a chain of reaction. One of the most tragic events that has ever happened that
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As Tom was clenching his fist tightly in a ball about to teach this fool a lesson‚ he had an instant flashback. This flashback took him back to his childhood. Tom’s flashback took him back to a very young part in his life where he was being bullied at school by some older boys. He got circled around and pushed to the ground and got his stuff taken away and his lunch money stolen from his own pants pockets. He got picked a ton when he was younger. His parents weren’t around much so he was pretty much
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