Effects of Differentiation on Enzymatic Activity Introduction HL-60 cells are capable of undergoing differentiation to induce different cell types. HL-60 cells can undergo morphological changes‚ changes in gene expression‚ and changes in protein synthesis. In the past weeks‚ we were able to conclude that HL-60 cells treated with DMSO and HL-60 cells treated with PMA will differentiate into granulocytes and monocytes upon treatment (1). We were also able to observe that both types of treated cells
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Abstract: The purpose of this lab is to synthesise acetylsalicylic acid (aspirin) by creating a reaction between acetic anhydride and salicylic acid. This was be accomplished through the use of recrystallization. Acetic anhydride and salicylic acid are mixed together‚ and then acidified by the addition of a few drops of concentrated sulfuric acid‚ which catalyzed the reaction. The percent yield is calculated to determine the effectiveness of the reaction in preparing the desired product (aspirin)
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rights. The law of nature protects people’s freedoms and keeps them under a code. While individuals are born inherently good‚ moderate greed is included in the law of nature as a way for “[man] to preserve himself” and to “preserve the rest of mankind.”2 The individual is put before all of mankind because mankind cannot survive without the individual. While the individual is the most important there are restrictions to protect each man’s freedom. The law of nature Hobbes creates a state of nature where
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spectrum of two different substances cannot be the same. Purpose/Hypothesis: 1. The purpose of the experiment was to determine the extinction coefficient for a solution of DCPIP. This was done by using the concentration‚ absorbance‚ wavelength values. 2. The purpose of the experiment was to determine the concentration of an unknown DCPIP sample by measuring the absorbance maximum of a specific concentration of DCPIP. 3. There was no hypothesis. We were merely trying to replicate the experiment. Procedure:
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Analysis‚ Synthesis‚ and Argumentative Essays The three types of essays mentioned in “Five Steps to a Five: AP English Language” are analysis‚ argumentative‚ and synthesis. An analysis essay is complied of different parts‚ it explains how the many parts can come together to produce a complete result. To have an argumentative essay you must acknowledge what you are defending and have sufficient and accurate information to support your claim. A synthesis essay should contain the main points of compared
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finish drying on a watch glass for the week. After a week take the product‚ and weigh it in a pre-weighed beaker. Calculate the percent yield. Test for Purity. Take 3 test tubes filled with water and dissolve a little bit of crystals in the first 2 tubes. Add 1 drop of 1% ferric chloride solution to each tube. Shake and note color. If impure‚ red to violet color will appear. Melting point can be used to test for purity. Melting point from a dry sample should be 135-136°C for pure aspirin. There
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The structure and synthesis of TPC is outlined in Scheme 1. Briefly‚ the compound 1 was synthesized as per literature procedure. 2 was synthesized by reacting 1-methy pyrazole-4-boronic pinacol ester with 1 by suzuki-coupling‚ the final product TPC was synthesized by treating with malononitrile in the presence of piperidine as a base and ethanol as solvent. The detailed synthesis procedure given in the experimental section. The structure and purity of TPC was unambiguously confirmed by 1H and 13C
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yield; 0.917g of 2-hydroxybenzoic acid (0.917/138) * 180 138 (the molar mass of 2-hydroxybenzoic acid) 180 (the molar mass of aspirin) Theoretical yield: 1.196g 3 d.p. Percentage yield is worked out by Actual yield of synthesised aspirin: 0.347g 0.347/1.196 * 100= 29 Percentage yield: 29% Melting Points Compound Began Melting (°C) Totally Melted (°C) Range (°C) Pure Aspirin 136 1-2 Crude Aspirin 125
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clumping together at the bottom of the test tube. See Table 2 for observations. The unknown had heat administered to it as well‚ in order to solidify the identity of the unknown;
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If I want to target the peptide to different compartments‚ I would use specific signal sequences or uptake-targeting sequences encoded into my peptide. Signal sequences contain information that will target the peptide to a particular organelle. The information is usually encoded within amino acid sequences of the protein itself‚ usually within the 20-50 amino acids. Also‚ each organelle has specific set of receptors that bind only to the specific signal sequences‚ thus making sure of target specificity
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