Determining Allele Frequencies of the PV92 Alu Element using DNA Isolated from Human Cheek Cells and PCR Amplification Background Alu elements are the most abundant repetitive elements in the human genome that have mobilized throughout primate genomes by retrotransposition over the past 65 million years ago from a 5’ to 3’ fusion of the 7SL RNA gene‚ to reach the present number of more than one million copies. Over the last few years‚ several lines of evidence demonstrated that these elements
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Identify an unknown carbonyl compound © KCl http://hk.geocities.com/fatherofchemistry Objective To classify a carbonyl compound by a simple test and to identify it by the precipitation of a derivative. Principle In this experiment‚ the unknown compound is either an aldehyde or a ketone from Table 90. In the first part‚ on undergoing Tollen’s & Fehling’s tests‚ ketone will have no reaction. In the second part‚ by preparing a derivative of the compound with 2‚4-dinitrophenylhydrazine
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Exp’t 84 Synthesis of Methyl Benzoate by Fisher Esterification from K. L. Williamson‚ Macroscale and Microscale Organic Experiments‚ 2nd Ed. 1994‚ Houghton Mifflin‚ Boston p385 Revised 10/15/03 Prelab Exercise: Give the detailed mechanism for the acid-catalyzed hydrolysis of methyl benzoate. Introduction: The ester group is an important functional group that can be synthesized in a number of different ways. The low-molecular-weight esters have very pleasant odors and indeed are the major components
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“The Synthesis of Zinc Chloride”‚ what a peculiar name for a lab that does not even involve synthesis. Synthesis‚ by definition‚ is when two elements are combined in a chemical reaction‚but that is not the case. In this lab we are combining zinc‚ an element‚ and hydrochloric acid‚ a compound‚ but combining a compound and an element is not synthesis‚ it is single replacement. We are taking zinc and hydrochloric acid and making zinc chloride and hydrogen‚ a single replacement‚ but what occurred during
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Determining Genotypic Frequencies for Alu Insertion Polymorphism at the PV92 Locus Introduction An Alu element is a short stretch of non-coding DNA found in primates. It gets its name from the single recognition site for the endonuclease Alu I‚ located near the middle of the Alu element. Alu elements are transposable DNA sequences that copy and insert themselves into new chromosome locations. They are regarded as “selfish DNA” because they do not encode protein and appear to only exist for their
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Junnel Metrillo Date Performed: October 27‚ 2014 2L Group 1 Date Submitted: November 3‚ 2014 Exercise 6 Dipeptide Sequence Determination To understand the chemical‚ structural and functional properties of a certain protein‚ it is important to determine its amino acid components and sequence. The differences in the composition and sequence of amino acids dictate
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Luminol solutions use oxidation reactions to create the bright‚ luminescent‚ blue emission. In order to discover which additive enhances that glow the most and for the longest‚ I studied the data of several tests with varying enhancers added to the luminol. The substances to be compared in this study are as follows: cysteine‚ Cu (II)‚ silver nanoparticles (AgNPs)‚ and zinc sulfide nanoparticles (ZnS-NPs). The first experiment focused on the reaction between alkaline luminol H2O2‚ the most common
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Thus a solution α-naphthol 1a‚ 4-methylbenzaldehyde 2a and tert-butyl isocyanide 3a in EtOH was magnetically stirred at 45 C for 12 hours under an oxygen atmosphere with an equimolar ratio of the three reactants. TLC monitoring of the reaction mixture exhibited formation of a new product‚ which was purified (Table 1‚ entry 1). Identification of its structure by NMR spectroscopy revealed that it was 2-(4-methylbenzoyl)-1-naphthyl N-(tert-butyl)carbamate (4a). Next‚ in order to improve the yield of
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In order to read DNA‚ it must be sequenced. This sequencing uses electrophoresis‚ a technique that separates sections of DNA that differ by a base. Electrophoresis used to be done manually‚ but was error prone and time consuming. Now‚ automatic sequencing machines are used. A technician begins the process by pouring gel between two glass plates that are set less than half a millimeter apart. After the gel is set up‚ DNA is put into each of the ninety-six lanes. The DNA sections then move through
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Based on the data from the riboflavin spectrum scan‚ the maximum absorbance wavelength for this compound is 446 nm. This was the point between 390 nm and 500 nm at which the absorbance value (0.72) was the highest. A blank tube that has the components of the solution being examined except for the compound of interest is then used in combination to provide an even more accurate reading. Then‚ by using Beer’s Law‚ the molar extinction coefficient for riboflavin was able to be calculated: 14‚400 L/(moles*cm)
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