The characteristic properties of slime can be explained by polymerisation reactions. Polymers can be explained most simply by the polymerisation of the molecule ethylene into polyethylene (see figure 1 below). Figure 1. Polymerisation of polyethylene from ethylene. In this example‚ the double bond between the carbon atoms is broken‚ allowing each carbon atom to form one more bond between another molecule. The double bond between the carbon atoms is weaker because the type of orbital is ‚ compared
Premium Polymer Monomer Plastic
The first step was to prepare 400 mL of an 0.1 M solution of NaOH. This was done by diluting from the 6 M solution that was provided. Next‚ 0.715g of KHP was weighed using the glazed paper and the triple beam balance. The KHP was then transferred to the 250 mL Erlenmeyer flask. Using a graduated cylinder‚ 50 mL of deionized water was measured and added to the flask. The KHP was dissolved in the water‚ and few drops of phenolphthalein were added. Moreover‚ the burette was rinsed with deionized water
Premium Erlenmeyer flask Sodium hydroxide Laboratory glassware
Specimen Preparation: Samples and all test reagent and kit were brought into room temperature. In 2ml eppendrof tube approximately 220mg stool sample were taken. Washing buffer was prepared by adding distilled water . Procedure for Purification of DNA from Stool sample: i. 220mg stool sample were collected in a 2ml tubes and placed it on ice. ii. Added 2ml Buffer ASL to each stool tube. Used pipet to wash the stool sample from the spoon while transferring the buffer. Vortexed continuously for 1minute
Premium Chemistry Protein Enzyme
Photolytic Degradation of Flupentixol-Melitracen Chapter One Introduction 1 Photolytic Degradation of Flupentixol-Melitracen 2 1. ANTIPSYCHOTIC 1.1 Antipsychotic An antipsychotic also known as neuroleptic is a tranquilizing psychiatric medication which is used to treat psychosis‚ schizophrenia and bipolar disorder. Now it is increasingly being used to treat the non-psychotic disorders. Psychosis disorder included delusions or hallucinations. Delusion is the pathological condition
Premium Schizophrenia Antipsychotic Psychosis
Throughout the data and the observations got from this experiment‚ there are some reasons which may affected the mass of the copper was recovered and also the percentage of yield. According to the law of conservation of matter‚ the mass of the copper will not change even though there are many chemical reactions happened which also mean that the mass of copper contained in solutions or precipitates remain the same as the copper in the beginning. And it is necessary to synthesize the various copper
Premium Chemistry Copper Chemical reaction
This experiment consisted of two parts. First‚ the absorption of each dye was evaluated by diluting (1 mg/mL standard) 2.0 mL of dye to 100 mL. The absorbance of each dye standard was measured and documented. The absorbance of the Gatorade was then measured by diluting the solution and pipetting 5.0 mL into a 25.0 mL volumetric flask. The absorption spectrum of the chosen drink was measured. Next‚ the purple Gatorade extraction procedure was carried out‚ beginning with pipetting 1 mL of 70% isopropanol
Premium
The purpose of the first week experiment was to determine the effect of Nitrophenyl phosphate concentration‚ the substrate‚ on the enzyme‚ acid phosphatase‚ reaction. In an enzyme reaction‚ there is substrate that binds to the active site of the enzyme and product is the end result. The enzyme is the catalyst for the reaction which means that it speeds up the process. Without enzymes‚ the majority of reactions would not occur because the activation energy level would be unattainable. With this in
Premium Chemistry Enzyme Chemical reaction
The lipase gene sequence from Alcaligenes sp. JG3 was translated into amino acid sequence using two methods‚ manual translation and ExPASy online software. By manual method‚ amino acid sequence was obtained via codon translation one by one as shown in Figure IV.7. The sequence reference used is the lipase from A. faecalis MOR02 due to having high similarity with the lipase sequence from strain JG3 during CLUSTAL alignment and the alignment of the deduced amino acid with the referred sequence was
Premium Enzyme Glucose Gene
two plates will just contain Luria Broth. Once again‚ one will contain plasmid and one will not. The research question for this experiment is: What is the difference in the transformation efficiencies between pFLO and pBLU. Before completing this lab‚ a first trial was done following the same procedure below but instead of pFLO‚ pBLU was used. It is believed that the transformations efficiencies should be similar due to the fact that both plasmids are
Premium Bacteria Escherichia coli
In this section we will analyze which steps were the most effective ones in recovering LDH (percent yield) and in purifying LDH (fold purification). As we can see looking at the Total Protein column on Table 3‚ the most effective step with regard to the percent of remaining protein removed was affinity chromatography because it was able to remove 98.6% of the remaining proteins. In comparison to 81.93% removed during the 65% ammonium sulfate precipitation and 81.3% during the size exclusion
Premium Water Revenue Balance sheet