The objective of these labs were to use sophisticated techniques to produce double-stranded RNA that was incubated with live Drosophila cells to inhibit the expression of our two genes of interest. The overall process of the four labs was to isolate and amplify DNA using the polymerase chain reaction with primers that contained gene-specific sequences to Thread or Dynamin-related protein 1 (drp-1)‚ along with T7 promoter site sequences. The amplified DNA was purified and both strands of the DNA
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Experiment Four: Organic Synthesis of Aspirin Abstract The purpose of this experiment is to synthesize a common organic product called acetylsalicylic acid (aspirin)‚ and to become familiar with the optimum conditions needed for successful yields. Aspirin is produced from an acid catalyzed reaction between salicylic acid with acetic anhydride. The crystalline aspirin is synthesized and purified by recrystallization‚ although there is not a hundred percent yield due to sources of error. Introduction
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Book of Esther Review 1. (Source Sheet 1‚ #5) For the first time‚ there are two major Jewish communities: one in Persia and one in Eretz Yisrael. There is a tension between the two communities. The community in Persia is assimilated and they were high up in status. The two communities do not mention one another; there is no mention of Shushan in any of the books of the people who lived in EY. In Persia‚ the Jews were very assimilated - the Jews intermarried and were economically very well off
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Experimental Synthesis of Aspirin and Melting Point Purity Analysis Donald Yeargin CH 222‚ Section 24221 Department of Chemistry Portland Community College Portland‚ OR Abstract The various methods available to synthesize aspirin lead to the need to examine and evaluate production efficiency and purity. The purpose of our experiment was to synthesize acetyl salicylic acid (aspirin) and then determine the relative purity of the synthesized sample by observing the melting point temperature
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All the reagents and solvents used for synthesizing the title compound were of Analar grade (Merck) and were used as such without further purification. The 2-naphthol derived Mannich base 1-((4-methylpiperazin-1-yl)(phenyl)methyl)naphthalen-2-ol (MPN) was synthesized according to the general procedure described in the literatures [13‚ 14]. The 2-naphthol (4.33 g‚ 0.03 mol) was dissolved in 30ml of ethanol and it was mixed with N-methylpiperizine (3.0 mL‚ 0.03 mol) by continuous stirrer at room
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Introduction The purpose of this experiment is to synthesize methyl nitrobenzoate from methyl benzoate‚ concentrated HNO3‚ and concentrated H2SO4 via an electrophilic aromatic substitution reaction. Reaction Procedures/ Observations Use a 50 ml beaker to cool about 6 ml of concentrated sulfuric acid in an ice water bath. Weigh the vial containing about 3 grams of methyl benzoate and add it to the cooled sulfuric acid. Next pour about 2 ml of sulfuric acid to the nitric acid in the vial
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14 may be a possible EEC. Moreover‚ the calculated compensation temperature by inverting the straight line slope‚ T comp = 57.9 ºC‚ is higher than the average experimental value‚ T exp = 32.5 ºC. This great difference between these two values of temperatures might reflect the existence of real EEC rather than experimental error [58]. Another evidence of EEC is the approximate constant ∆G # with a significant change in both ∆H # and ∆S # . Finally‚ EEC was also investigated by using
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The data we collected and the calculations we performed demonstrated that the color of a given food dye depended on which light was transmitted in the visible spectrum and which light was absorbed. A quantitative measurement of the absorbance spectrum of each food dye was obtained through spectrophotometry.1 Through this method‚ we determined that the wavelength of maximum absorbance of a given food dye was directly correlated to the color of that food dye. Further‚ darker food dyes absorbed more
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Name Period Date The Synthesis of Aspirin Background Pharmaceutical research labs perform chemical reactions between organic molecules that contain mostly carbon. Since the atoms in organic molecules tend to be nonmetals‚ then the bonds that hold them together are covalent bonds. Only a few elements on the periodic table are nonmetals: C‚ N‚ O‚ S‚ Cl‚ and F. This small number of elements can be bonded together in different quantities‚ bonding types (single‚ double‚ triple bonds)‚ and structural
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I. INTRODUCTION Synthesis Acetylsalicylic acid is an acetyl derivative of salicylic acid which appears as a white and crystalline and a weakly acidic substance with a melting point of 135°C and is also known to be aspirin. It is one of the most widely used drugs that can lower fever and is used as a painkiller. According to (Escobel‚ 2011)‚ Aspirin is synthesized through the reaction of salicylic acid with acetyl anhydride that causes a chemical reaction that turns salicylic acid’s hydroxyl group
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