is associated with lactase persistence is the located upstream of the LCT gene. In this mutation a single nucleotide polymorphism changes a cytosine into a thymine that then can be detected using the polymerase chain reaction technique (Biology 225 lab manual‚ S2017). In this experiment amplification Refractory Mutation System PCR was used to detect this nucleotide change. Two primers were used in two different PCR reactions‚ one to detect the wild type allele and the other to detect the mutant allele
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PRE-LAB The purpose of the lab is to determine the moles of hydrogen gas and then molar mass of the metal. First‚ the metal assigned to each group must be weighed out in order to ensure the hydrogen gas produced will not exceed the capacity for the gas measuring tube. Then‚ copper will be wrapped around the metal leaving 6-8cm at the end for a handle. The end wire will be bent into a hook to keep the sample at the bottom of the tube and increase the oxidation rate. A ring stand will need to be
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First‚ the synthesis of the coordination compound‚ KaFeb(ox)cdH2O‚ needed to be carried out. This was achieved by weighing out 8.29 grams of K2C2O4 with a Dial-a-gram balance. Once measured‚ the 8.29 grams K2C2O4 were placed in a 125 mL Erlenmeyer flask. 25 mL of deionized water were then added to the 125 mL flask and stirred with the K2C2O4 until complete dissolution was achieved. Next‚ 4.11 grams of FeCl3 were weighed out using the balance previously mentioned. Once the 4.11 grams were measured
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Lab #28 Conservation of Mass Ashleigh Bublinec Serena Contreras
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5.4. Synthesis of Poly L-Lactide (PLA) PLA was synthesized by the ring opening polymerization of L-Lactide in presence of stannous octoate. L-Lactide (1.44 g (0.01 mol)) was dissolved in 20 mL of toluene; 1.5 mg of stannous octoate was added to this solution. The
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This analysis concluded that the unknown was ethylene glycol. This was discovered as the gas was heated to 90 degrees Celsius causing the liquid unknown to evaporate into a gas while recording the mass‚ volume‚ temperature‚ and volume. This provided the mass of 1.6 grams which was found by subtracting the mass of the Erlenmeyer flask‚ aluminum cover‚ and rubber band from the mass of the Erlenmeyer flask‚ aluminum cover and rubber band and unknown gas after heating. Then the temperature of the gas
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Figure 6: Imatinib The first imatinib drug synthesis was done by Zimmermann (figure 7) in 1993‚ he used a chain of the reaction to reach to imatinib but the important part is that both guanidinium nitrate 7 and aminopyrimidine 8 are precipitated from the respective reaction mixtures by the addition of Figure 7: imatinib synthesis by Zimmermann insolubilizing solvents‚ so we got a condensation reaction between guanidinium salt 7 with enone “9” to form the aminopyrimidine “8” then “8” is
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Tie-Dye Grignard Synthesis Abstract: 4-Bromo-N‚N-dimethylaniline underwent a Grignard reaction with diethyl carbonate to produce a type of the tie-dye chemical triarylmethane. This specific triarylmethane produces a vivid crystal violet color when dyed. The experiment was first heated under reflux to produce the necessary Grignard reagent as a grey liquid. It was then reacted with diethyl carbonate and hydrochloric acid to produce crystal violet. The resulting chemical was very absorbent to
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The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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blue green dihydrate. Copper (II) Chloride is highly soluble in water and will produce a blue solution. solutioAluminum is the compound that has a chemical formula of Al. This is a silver solid that can be easily formed‚ machined‚ or cast. In this lab‚ we will be finding the limiting reactant between Copper(II) Chloride and Aluminum. The limiting reactant is‚ reactant is‚ the reactant in a chemical reactant that limit’s the amount of the product that can be formed. We will also need to find the molarity
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