Synthesis of Isopentyl Acetate (Banana Oil) Using A Fischer Esterification Reaction Purpose: The purpose of this experiment is to synthesize Isopentyl Acetate using a Fischer esterification reaction. Fischer esterification is an acid-catalyzed condensation of an alcohol and a carboxylic acid‚ yielding an ester and water. Isopentyl Acetate has the scent of banana oil‚ once synthesized it will be purified by distillation. Then the analysis of the sample using H NMR and IR will determine purity
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Organic Lab 2 February 14‚ 2009 Experiment 4: Borohydride Reduction of 2-Methylcyclohexanone Introduction: When learning about reduction reactions‚ we know that the addition of nucleophilic hydride anion (H-) to the carbonyl group will in turn result in the reduction of aldehydes to primary alcohols. Borohydride‚ and Lithuim aluminum hydride are commonly used as reducing agents. We can note that both of these reagents have a (H-) anion‚ hence it will be a powerful base and also take the
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The following paragraph describes the procedures and steps taken on day one of the experiment‚ which focused entirely on the synthesis of the isomer based on Protocol B. Certain chemical agents used on day one have both health and safety risks‚ specific details regarding these risks will be explained in more detail in the following procedures‚ but if unfamiliar with any of these
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Determining Allele Frequencies of the PV92 Alu Element using DNA Isolated from Human Cheek Cells and PCR Amplification Background Alu elements are the most abundant repetitive elements in the human genome that have mobilized throughout primate genomes by retrotransposition over the past 65 million years ago from a 5’ to 3’ fusion of the 7SL RNA gene‚ to reach the present number of more than one million copies. Over the last few years‚ several lines of evidence demonstrated that these elements
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Identify an unknown carbonyl compound © KCl http://hk.geocities.com/fatherofchemistry Objective To classify a carbonyl compound by a simple test and to identify it by the precipitation of a derivative. Principle In this experiment‚ the unknown compound is either an aldehyde or a ketone from Table 90. In the first part‚ on undergoing Tollen’s & Fehling’s tests‚ ketone will have no reaction. In the second part‚ by preparing a derivative of the compound with 2‚4-dinitrophenylhydrazine
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Determining Genotypic Frequencies for Alu Insertion Polymorphism at the PV92 Locus Introduction An Alu element is a short stretch of non-coding DNA found in primates. It gets its name from the single recognition site for the endonuclease Alu I‚ located near the middle of the Alu element. Alu elements are transposable DNA sequences that copy and insert themselves into new chromosome locations. They are regarded as “selfish DNA” because they do not encode protein and appear to only exist for their
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Junnel Metrillo Date Performed: October 27‚ 2014 2L Group 1 Date Submitted: November 3‚ 2014 Exercise 6 Dipeptide Sequence Determination To understand the chemical‚ structural and functional properties of a certain protein‚ it is important to determine its amino acid components and sequence. The differences in the composition and sequence of amino acids dictate
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Luminol solutions use oxidation reactions to create the bright‚ luminescent‚ blue emission. In order to discover which additive enhances that glow the most and for the longest‚ I studied the data of several tests with varying enhancers added to the luminol. The substances to be compared in this study are as follows: cysteine‚ Cu (II)‚ silver nanoparticles (AgNPs)‚ and zinc sulfide nanoparticles (ZnS-NPs). The first experiment focused on the reaction between alkaline luminol H2O2‚ the most common
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Section 2 EXPERIMENT: DNA & Protein Synthesis Exercise 1 – Modeling DNA 1. List the four bases which are found in DNA. (1 pt) The four bases found in DNA are cytosine‚ adenine‚ guanine and thymine. 2. Fit any six nucleotides together to form a row‚ then list the six nucleotides in the order you used them. Work with your model pieces and try fitting the bases together to make a double strand as shown in Figure 9 of the lab manual. Which nucleotides form pairs
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Thus a solution α-naphthol 1a‚ 4-methylbenzaldehyde 2a and tert-butyl isocyanide 3a in EtOH was magnetically stirred at 45 C for 12 hours under an oxygen atmosphere with an equimolar ratio of the three reactants. TLC monitoring of the reaction mixture exhibited formation of a new product‚ which was purified (Table 1‚ entry 1). Identification of its structure by NMR spectroscopy revealed that it was 2-(4-methylbenzoyl)-1-naphthyl N-(tert-butyl)carbamate (4a). Next‚ in order to improve the yield of
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