Title: Prep of t-Butyl Chloride via SN1 Reaction Purpose: The purpose of this experiment is to synthesize tert-butyl chloride via an SN1 reaction. t-Butyl Chloride was synthesized from t-Butyl Alcohol using hydrochloric acid in separatory funnel; isolation of t-Butyl Chloride was done under distillation conditions. The experiment resulted in 8.29grams of purified compound‚ which is a 66.27 percent yield. Procedure: As per handout with changes Equation: Mechanism: Results: (Scan
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is associated with lactase persistence is the located upstream of the LCT gene. In this mutation a single nucleotide polymorphism changes a cytosine into a thymine that then can be detected using the polymerase chain reaction technique (Biology 225 lab manual‚ S2017). In this experiment amplification Refractory Mutation System PCR was used to detect this nucleotide change. Two primers were used in two different PCR reactions‚ one to detect the wild type allele and the other to detect the mutant allele
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PRE-LAB The purpose of the lab is to determine the moles of hydrogen gas and then molar mass of the metal. First‚ the metal assigned to each group must be weighed out in order to ensure the hydrogen gas produced will not exceed the capacity for the gas measuring tube. Then‚ copper will be wrapped around the metal leaving 6-8cm at the end for a handle. The end wire will be bent into a hook to keep the sample at the bottom of the tube and increase the oxidation rate. A ring stand will need to be
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Lab #28 Conservation of Mass Ashleigh Bublinec Serena Contreras
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This analysis concluded that the unknown was ethylene glycol. This was discovered as the gas was heated to 90 degrees Celsius causing the liquid unknown to evaporate into a gas while recording the mass‚ volume‚ temperature‚ and volume. This provided the mass of 1.6 grams which was found by subtracting the mass of the Erlenmeyer flask‚ aluminum cover‚ and rubber band from the mass of the Erlenmeyer flask‚ aluminum cover and rubber band and unknown gas after heating. Then the temperature of the gas
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Name: Bhumiben Shah Date: 31st Jan ‘13 EXPERIMENT # 2 RECRYSTALLIZATION; FILTRATION OBJECTIVES: 1. To perform recrystallization and filtration of given impure organic compound. 2. To purify impure acetanilide using reflux condenser apparatus and Hirsch funnel filtration. 3. To determine percentage recovery of pure material (which is)‚ purified by recrystallization and filtration. SAFETY PRECAUTIONS: 1. Operate the aspirator with the maximum water-flow using a stop cock to
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Introduction: In this experiment‚ when vinyl strip and acetate strip is rubbed by tissue‚ it will charge the vinyl strip negatively and acetate strip positively caused by the transfer of electron between the strip and tissue. If molecules have non zero bond dipole‚ because polar molecule must have a non-zero molecular dipole where electron spend more of their time closer to one atomic nucleus than the other and make one end partially positive or negative than the other end. Different charge will
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African American Studies Final Paper The first reading I chose was “A’n’t I a woman” by Soujourner Truth. Soujourner Truth was originally named Isabella Baumfree at birth. Truth was born into slavery on November 26‚ 1883 in New York where she was later freed by the New York State Emancipation Act of 1827. This was written ten years before the Civil war and at this point‚ African Americans began fighting for their freedom. “A’n’t I a Woman?” was first heard during a famous speech given at a women’s
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The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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Junnel Metrillo Date Performed: October 27‚ 2014 2L Group 1 Date Submitted: November 3‚ 2014 Exercise 6 Dipeptide Sequence Determination To understand the chemical‚ structural and functional properties of a certain protein‚ it is important to determine its amino acid components and sequence. The differences in the composition and sequence of amino acids dictate
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