CHEM 1474-E01 Chemistry for Everyday WEEK5 Lab Report Spring 2017 Instructor: Kyoung Nan Kim Office: SI3076 Email: kyoung.kim@ucdenver.edu Office hour: M/W 1:00p-2:30p (SI3076) T/Th 12:30p-1:30p (SI3076) • All communication should be done via school email (xxxx@ucdenver.edu) • The work will be submitted through canvas assignments. You can simply upload doc/pdf files under “Lab report” assignment tap. • Review: Ch2‚ Ch3.5‚ Ch 4.2‚ Ch6.1 and Ch7.1-Ch 7.3 in your text book (21st
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system to isolate the alum. 2 Al(s) + 2 KOH + 22 H2O + 4 H2SO4 → 2 KAl(SO4)2●12H2O(s) + 3 H2(g) Its composition was analyzed using two precipitation tests and two flame tests. Three methods for growing crystals were then set up for observation next lab. Theoretical yield and percent yield: The theoretical yield for alum was 4.55g and the percent yield was 139%. The percent yield was above 100% because the alum was saturated with ethanol and water. KOH test: The KOH test identified the presence of
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Section 2 EXPERIMENT: DNA & Protein Synthesis Exercise 1 – Modeling DNA 1. List the four bases which are found in DNA. (1 pt) The four bases found in DNA are cytosine‚ adenine‚ guanine and thymine. 2. Fit any six nucleotides together to form a row‚ then list the six nucleotides in the order you used them. Work with your model pieces and try fitting the bases together to make a double strand as shown in Figure 9 of the lab manual. Which nucleotides form pairs
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amount of a chemically modified base is added. The four different batches are for the four different bases; a modified T goes in one batch‚ modified A in another‚ modified G in another‚ and modified C in the last. The modified bases cause the DNA sections to stop growing; The growing stops on the modified base that is inserted. The modified T will only have pieces that end with T‚ and so on. This causes the sections in the different batches to be different lengths. A florescent dye is also added into
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The lipase gene sequence from Alcaligenes sp. JG3 was translated into amino acid sequence using two methods‚ manual translation and ExPASy online software. By manual method‚ amino acid sequence was obtained via codon translation one by one as shown in Figure IV.7. The sequence reference used is the lipase from A. faecalis MOR02 due to having high similarity with the lipase sequence from strain JG3 during CLUSTAL alignment and the alignment of the deduced amino acid with the referred sequence was
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Abstract – The synthesis of 4-hydroxy-4‚4-diphenylbutn-2-one was a three week multi-step project. The steps involved protecting the ketone of ethyl acetoacetate with a ketal group‚ followed by preparation and reaction with Grignard‚ then purification by extraction and recrystallization. The yield was 78.5%‚ but there are impurities present suggested by 1H-NMR‚ IR‚ and TLC. However‚ the results do indicate the desired hydroxyketone was formed. Introduction: The purpose of this multi-step lab was to synthesize
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Empirical Formula of Copper (II) Chloride Gabriella Jane Lukas B1401404 HELP University Empirical Formula of Copper (II) Chloride Objectives 1. To validate that the empirical formula of copper (II) chloride is . 2. To calculate the percent composition of copper in copper (II) chloride. 3. To illustrate the Law of Constant Composition in copper (II) chloride. 4. To study the reaction between copper (II) chloride solution and aluminium metal. Introduction One of the most fundamental statements
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Pat 1 Synthesis of 4-aryl-6-indolylpyridine-2-carbonitriles and evaluation of their biological activity Synthesis of 6-indolyl-4-aryl-3-cyanopyridine-2-one (1a-e) Employing the MCRs approach and microwave irradiation‚ an equimolar amounts of aromatic aldehyde‚ 3-acetylindole‚ ethyl cyanoacetate were reacted with excess of ammonium acetate in one-pot‚ reaction‚ under the microwave irradiation‚ afforded a novel series of 4-aryl-6-indolyl-nicotinonitrile-2-one derivatives 1a-e. Ethylene glycol was
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Introduction In this lab‚ caproic acid was synthesized in a multi-step process that involved the synthesis of three intermediates – diethyl n-butylmalonate‚ potassium n-butylmalonate‚ and n-butyl malonic acid respectively. An IR was used to characterize the starting material‚ n-bromobutane‚ and the first intermediate‚ diethyl n-butylmalonate; while IR and NMR were used to characterize the final product‚ caproic acid. Reactions‚ Mechanism and Theory Caproic acid a.k.a n-hexanoic acid is a carboxylic
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Organic Lab 2 February 14‚ 2009 Experiment 4: Borohydride Reduction of 2-Methylcyclohexanone Introduction: When learning about reduction reactions‚ we know that the addition of nucleophilic hydride anion (H-) to the carbonyl group will in turn result in the reduction of aldehydes to primary alcohols. Borohydride‚ and Lithuim aluminum hydride are commonly used as reducing agents. We can note that both of these reagents have a (H-) anion‚ hence it will be a powerful base and also take the
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