The experiment consists of 5 steps—Inoculation‚ fermentation‚ cell lysis‚ inclusion body solubilization‚ and peptide purification. In the first two steps‚ E coli with Snake 6 peptides is cultivated. Ultraviolet–visible spectroscopy (UV-vis) is used to quantify bacteria through detection of their optical density (O.D.). After reaching a certain concentration in the fermentation batch‚ inclusion bodies (IBs) are extracted from these E coli by adding Bugbuster lysis solution and lysozyme. Polyacrylamide
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MATERIAL AND METHODS Recombinant protein production The recombinant T-Rp3 was expressed in E. coli BL21 (DE3) as previously described (Favaro et al.‚ 2014) and purified by a single step His-based affinity chromatography in an ÄKTA purifier FPLC (GE Healthcare). Protein was then dialyzed against 50 mM MES buffer (pH 6‚0) overnight at room temperature‚ frozen in liquid nitrogen and stored at -80 ˚C after 0.22 μm pore membrane filtration. Evaluation of DNA-protein interaction by gel retardation
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Objective: The objective of this lab is to determine and analyze the many chemical qualities of copper ‚ by using many solutions that enable copper to undergo chemical changes that will take copper from a metal back to its original state. This is done using cooper‚ Concentrated HNO3‚ 6 M NaOH ‚ Bunsen burner‚ 6 M H2SO4‚ Mg ribbon‚ and two test tubes. The techniques used in this lab which is cleaning glassware‚ disposing of chemicals ‚ measuring mass ‚ centrifugation ‚ venting gases‚ and test tube
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Gringard Synthesis detailed procedure and scheme of the apparatus any changes to the original procedure‚ actual masses‚ and obs yield calculations and mp Discussion outline Grignard rxn (what is it used for‚ why important‚ the mechanism) Reaction set up (important details) How can the rxn be activated Second step: rxn of the Grignard reagent with acetophenone‚ quenching with ammonium chloride Isolation of the product‚ identification Possible or actual sources of error Part one of our experiment
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(NH2CH2CH2NH2) that formed [Co(en)2(H2O)2]Cl. Then the [Co(en)2(H2O)2]Cl was reacted with H2O2 to change the cobalt’s charge to cobalt(III) from cobalt(II). Finally HCl was added into the solution to form [Co(en)2Cl2]Cl. The percent yield was 45.51%.To synthesis trans isomers of [Co(en)2Cl2]Cl‚ distilled water was first mixed with CoCl2▪6H2O‚ the solution color was changed to wine color. After ethlenediamine (NH2CH2CH2NH2) was added‚ the color of the solution changed to blood color‚ and kept its color until
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The tert-butyl group has been broadly used to protect functional groups in natural product synthesis such as amino acid‚ peptide and protein chemistry [1]. The tert-butoxycarbonyl (BOC) group is known as protecting group for amides in organic synthesis. The introduction of BOC group improves the solubility of organic precursors and their processability in organic synthesizes [2]. The methyl substituted pyridine derivatives were widely reported for their various biological activities such as anxiolytic
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1. What command can you enter in the Search box to launch Device Manager? Devmgmt.msc 2. Which is faster‚ an eSATA-600 port or a FireWire 800 port? eSATA-600r 3. What is the speed for Hi-Speed USB? 480 Mbps 4. How many times faster is a Hi-Speed USB port than an Original USB port running at 12 Mbps? 468Mbps 5. Which is faster‚ USB 3.0 or eSATA 600? eSATA 600 6. Which is faster‚ Wi-Fi 802.11n or Bluetooth? Wi-Fi 802.111n 7. How many pins does a FireWire 800 port have? 9-pin 8.
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White’s purpose of writing is to provide a long overdue examination of female slavery‚ ending long held myths and exemplifying the distinctive struggles that slave women faced in their day to day survival. Deborah Gray White’s book‚ Ar’n’t I a Woman? categorizes black women in the context of the two dogmas they faced in the antebellum South—the Southern feminine model of the dependent‚ physically inert female‚ and the tougher imagery of tough labor and dehumanization that was experienced daily in
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2.4.General procedure for the synthesis of 2‚3-dihydriquinazolin-4(1H) ones derivatives: A stirred mixture of isatoic anhydride (1 mmol)‚ amines containing 2-aminobenzothiazole or 2-amino benzimidazole (1.1mmol)‚ aromatic aldehydes (1 mmol) and 0.045 g of H-ZSM-5 nanozeolite was reacted in an oil bath at 100 oC for the appropriated times. Completion of the reaction was indicated by TLC. After completion of reaction‚ the mixture was cooled to room temperature. The solid residue was dissolved in hot
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IN-N-OUT BURGER You may be thinking‚ “Hey‚ what’s the big deal about IN-N-OUT that you have to write an article about it? But if that is what you’re thinking‚ then you are likely to have one or more or these great restaurants in your town. However‚ not everybody visiting LasVegas has an IN-N-OUT BURGER back home‚ but they are likely to have heard of this fast food chain. A regional burger chain IN-N-OUT BURGER is not found throughout the Untied States‚ and this is due to several reasons‚ but
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