● Cobalt(II) Chloride Hexahydrate CoCl2.6H2O‚ was weighed in a weighing boat. The Cobalt(II) Chloride Hexahydrate was transferred into a 100mL volumetric flask dissolved in water. ● 25 mL of the dissolved was measured‚ using a graduated cylinder and few of the dissolved Cobalt (II) Chloride Hexahydrate‚ was poured into test tube. The test tube was labeled with a tape‚ to signify 25 mL and the spectrophotometer was used to record the U-V visible ABS (absorbance spectrum) with the wavelength of 515
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Nucleophilic Substitution | Synthesis of n-Butyl Bromide and t-Pentyl Chloride | | Jessica | [Pick the date] | Abstract The synthesis of the alkyl halide n-Butyl Bromide from alcohol is the foundation for the experiment. During the isolation of the n-butyl bromide‚ the crude product is washed with sulfuric acid‚ water‚ and sodium bicarbonate to remove any remaining acid or n-butyl alcohol. The primary alkyl halide halide n-butyl bromide is prepared by allowing n-butyl alcohol to react with
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“The Synthesis of Zinc Chloride”‚ what a peculiar name for a lab that does not even involve synthesis. Synthesis‚ by definition‚ is when two elements are combined in a chemical reaction‚but that is not the case. In this lab we are combining zinc‚ an element‚ and hydrochloric acid‚ a compound‚ but combining a compound and an element is not synthesis‚ it is single replacement. We are taking zinc and hydrochloric acid and making zinc chloride and hydrogen‚ a single replacement‚ but what occurred during
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In order to find the formula of the copper chloride hydrate‚ we had to separate the compound to find the mass of water and copper. To begin this process‚ we evaporated the water and created an anhydrous compound‚ meaning we were left with only CuxCly. By calculating the weight of both the anhydrous and the hydrated compounds‚ we could conclude that the difference in the weights was the weight of the H2O. From this we were able to calculate the percent composition of CuxCly and H2O (see Calculations:
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Preparation of t-Butyl-Chloride March 8 & 15‚ 2012 Theory: Alkyl halides can be synthesized when alcohols react with hydrogen halides. An alkyl halide is a halogen-substituted alkane‚ and a hydrogen halide is a compound consisting of a hydrogen bonded to a halogen (H-X). Alkyl halides are classified as primary‚ secondary‚ or tertiary depending on the number of alkyl substituents directly attached to the carbon bearing the halogen atom. The purpose of this laboratory experiment was to prepare t-butyl-chloride
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In the experiment‚ we tested a sodium chloride solution. Along with the tested solution‚ control groups (water and sodium phosphate) were used to be help understand whether or not NaCl was a buffer. Water was the negative control group and sodium phosphate was the positive control group. If NaCl was a buffer than the pH would be stabled as the sodium phosphate buffer. If NaCl was not a buffer than the pH would fluctuate like the negative control‚ water. During the first trial and prior to the drops
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The leaves‚ bark and fruit of the T. conophorum have been reported to treat a number of diseases including giddiness‚ toothache‚ eczema‚ pruritus‚ psoriasis‚ common cold‚ prostate cancer and male infertility [8]. However‚ the fact that an herbal preparation is of natural origin does not necessarily make it safe. The active ingredients of plant extracts are chemicals like those of synthetic or purified drugs. In low amounts‚ they may be ineffective‚ while in the right amounts‚ they may prove beneficial
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Abstract A Friedel-Crafts alkylation was performed by adding t-butyl alcohol to p-dimethoxybenzene in order to produce 1‚4-di-t-butyl-2‚5-dimethoxybenzene. This reaction yielded 0.009g of 1‚4-di-t-butyl-2‚5-dimethoxybenzene having a percent yield of 5%‚ and a melting point range of 54.8°C-56.9°C. Introduction This reaction is designed to put functional groups onto aromatic rings. This is done through an electrophilic aromatic substitution where a positive species is strong enough to pull electrons
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Empirical Formula of Copper (II) Chloride Gabriella Jane Lukas B1401404 HELP University Empirical Formula of Copper (II) Chloride Objectives 1. To validate that the empirical formula of copper (II) chloride is . 2. To calculate the percent composition of copper in copper (II) chloride. 3. To illustrate the Law of Constant Composition in copper (II) chloride. 4. To study the reaction between copper (II) chloride solution and aluminium metal. Introduction One of the most fundamental statements
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MATERIAL AND METHODS Recombinant protein production The recombinant T-Rp3 was expressed in E. coli BL21 (DE3) as previously described (Favaro et al.‚ 2014) and purified by a single step His-based affinity chromatography in an ÄKTA purifier FPLC (GE Healthcare). Protein was then dialyzed against 50 mM MES buffer (pH 6‚0) overnight at room temperature‚ frozen in liquid nitrogen and stored at -80 ˚C after 0.22 μm pore membrane filtration. Evaluation of DNA-protein interaction by gel retardation
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