Experiment 2: Estimation of protein concentration Introduction Protein assays are designed to measure the total protein in a solution. Protein assays are quantitative if the protein to be assayed is available in sufficient quantity such that one is able to use it to create a standard curve. If this cannot be achieved‚ then a standard protein‚ such as albumin‚ may be used for a standard curve with the understanding that the results on the unknown protein are semi-quantitative. Since most proteins
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-APPLICATION OF ENZYMES IN ORGANIC CHEMISTRY Why enzymes in organic synthesis? | Alternative to chemical methods: * High region- and stereoselectivity * Milder reaction conditions * Environmentally more friendly | Which enzyme(s)? | OxidoreductasesHydrolasesLyasesIsomerases | Which reaction system? | AqueousAqueous and water-miscible organic solventAqueous and water-immiscible organic solventPure organic solventOther solvents (supercritical fluids‚ ionic liquids) | Which ‘chemistry’
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Håvard Devold Oil and gas production handbook An introduction to oil and gas production ISBN 978-82-997886-1-8 PREFACE This handbook has been compiled for readers with an interest in the oil and gas production industry. It is an overview of the main processes and equipment. When we searched for a suitable introduction to be used for new engineers‚ I discovered that much of the equipment is described in standards‚ equipment manuals and project documentation. But little material was
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Table of Specifications A Table of Specifications is a blueprint for an objective selected response assessment. The purpose is to coordinate the assessment questions with the time spent on any particular content area‚ the objectives of the unit being taught‚ and the level of critical thinking required by the objectives or state standards. The use of a Table of Specifications is to increase the validity and quality of objective type assessments. The teacher should know in advance specifically
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2014 Hogan 5th Period English I Auschwitz: The Death Camp Thesis: Built by the Nazis as both a concentration and a death camp‚ Auschwitz was the largest of the Nazi camps‚ the most diverse and intricate camp of all‚ and the main center for the death of Jews during the Holocaust. Outline I. Largest of Nazi Death Camps A. Consisted of three camps B. Thirty-seven sub camps C. Seven gas chambers and crematories II. Most diverse and intricate camp A. Decorations made the camp look “fun”
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Equipment‚ and Methods Sixth Edition AGGREGATE PRODUCTION • A. J. Clark School of Engineering •Department of Civil and Environmental Engineering 14 By Dr. Ibrahim Assakkaf ENCE 420 – Construction Equipment and Methods Spring 2003 Department of Civil and Environmental Engineering University of Maryland‚ College Park CHAPTER 14. AGGREGATE PRODUCTION Slide No. 1 ENCE 420 ©Assakkaf PRODUCTION OF CRUSHEDSTONE AGGREGATE The production of crushed-stone aggregate involves: Drilling Blasting
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SOLUTION AND CONCENTRATION A solution is a homogeneous mixture‚ made up of a solute dissolved in a solvent. Ex. In a water (aqueous) solution of sodium chloride‚ the sodium chloride is the solute and the water is the solvent. Solute – the component that is dissolved or is the least abundant component in the solution. Ex. Sugar‚ salt Solvent – the dissolving agent or the most abundant component in the solution. Ex. Water Common Types of Solution State of Solution State of Solute
Free Concentration
besides the methods of producing it is incomplex and it can give big impact to the world. II Body A Ethanol is widely recognized these days as a very promising alternative source of energy (Low & Isserman‚ 2009) 1 Ethanol produced from waste products is blended with gasoline to produce bio-fuels and it is broadly used nowadays (Rangasamy et al‚2011) a Ethanol can reduce our dependence
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Boyle’s Law - Solutions 1) If I have 5.6 liters of gas in a piston at a pressure of 1.5 atm and compress the gas until its volume is 4.8 L‚ what will the new pressure inside the piston be? P1V1 = P2V2 (1.5 atm)(5.6 L) = (x)(4.8 L) x = 1.8 atm 2) I have added 15 L of air to a balloon at sea level (1.0 atm). If I take the balloon with me to Denver‚ where the air pressure is 0.85 atm‚ what will the new volume of the balloon be? P1V1 = P2V2 (1.0 atm)(15 L) = (0.85 atm)(x) x = 18
Free Pressure Ideal gas law Gas
on the Save a Copy button on the panel above to save your report) Activity: Name: Instructor: Date: Enzyme Activity cheryl yelton November 30‚ 2014 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3
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