Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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because using cost-distance it is near to impossible to acquire the same effect as with the buffers. Another asset of the buffer method is that‚ after its creation‚ the buffers can be used or transformed easily into something else to answer a slightly different question as at the moment the buffers are rather bare‚ but it would be quite easy to transform them into multiple buffers‚ or even use the buffer zones as a start for other analysis like the
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2% of soluble starch • 250-mL volumetric flask • 500-mL volumetric flask • 0.03 M H3AsO3 • 0.1 M KIO3 • 0.2 M KI Procedure Buffer A and buffer B were prepared first with the concentration of Buffer A being roughly half that of Buffer B. The preparation of Buffer A is as follows: 100 mL of 0.75 M NaAc solution‚ 100 mL of 0.22 M HAc
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I. Introduction The purpose of this experiment is to determine the pH values of acids‚ bases‚ and buffers of distilled water and 10.0 buffer using measured concentrations of Sodium hydroxide (NaOH) and/or Hydrochloric acid (HCl). Acid is a compound typically having a bitter taste and capable of nullifying alkalis and releases hydrogen ion when added to a solution‚ or containing an atom that can accept a pair of electrons from a base (McKinley‚ Dean O’Loughlin‚ & Stouter Bidle‚ 2016). Bases are water-soluble
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DNA ligase. All the reaction components such as ligation buffer‚ DNA insert‚ pGEM®-T‚ and T4 DNA ligase is mixed by gentle pipetting. The 2X rapid ligation buffer is used in this experiment to saving the ligation time. This ligation buffer contains ATP. ATP is used to catalyze the formation of phosphodiester bond and the reaction of restriction enzyme buffer. The recommended time and temperature of incubation when using 2X rapid ligation buffer are one hour at room temperature (24°C) and overnight
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and Gray‚ p.302). Then‚ Pinyarat explained that the activity durations had gotten squeezed down to less than the 50 percent guideline and that the estimates were impossible. Pinyarat also mentioned that she was allowed to put in a big enough project buffer and other members of the firm had no idea why management would use CCPM scheduling in these projects (Larson and Gray‚ p.303). It is believed that project managers who estimate activity times provide an estimate that has about an 80 to 90 percent
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What is a pH buffer? A buffer is a solution in which the acid and the base are being controlled. By adding a certain amount of the buffer‚ it can easily change the pH level of the solution. Its pH changes very little when a small amount of strong acid or base is added to it and thus it is used to prevent changes in the pH of a solution. These buffer solutions are very useful in different chemical applications in which the pH value are being kept at a constant level. Every buffer that is made has
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Hydroxyquinone Oxidation/Reduction Pink ³ Brown E+S + [ES] = E+P Enzyme Reaction Hypothesis: If there is an increase in enzyme concentration‚ an increase in reaction temperature‚ or an increase in buffer pH‚ then greater intensity in a given reaction will be experienced‚ resulting in greater manipulation of the final substrate product; measured by the extent of substrate color change. Greater manipulation of substrate when introduced into stronger
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Finish (EF): Latest possible point in time on which a task can finish.Early Start and finish dates are calculated by means of Forward Pass and Late Start and Late Finishdates are calculated by means of Backward Pass.Many Tasks have some amount of buffer added to them referred as Slack Time or Float. Float time isamount of time a task can slip before it delays project schedule.
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phosphate buffer pH6 containing the enzyme: polyphenol-oxidase‚ was used as the variable being tested in this experiment. You may refer to the Enzymatic Reactions Biology 21 Lab Manual. Santa Monica College by Logan‚ R. 2003 to see how the potatoes extract in phosphate buffer was made. We began this experiment by measuring seven constant amounts of 1ml of 0.1% catechol using a 1 mL pipet into each seven cuvettes. The catechol is our substrate solution. Next‚ different amounts of phosphate buffer ph.6
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