Preformulation activities range from supporting identification of new active agents to characterizing physical properties necessary for the design of dosage forms. Critical information provided during the preformulation can enhance the rapid and successful introduction of new therapeutic entities for humans. Hence‚ preformulation studies are essential to characterize drugs for proper designing of drug delivery systems. The preformulation studies‚ which were performed in this project‚ include – identification
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LAB 6. ACIDS AND BASES: PH AND BUFFERS PURPOSE: To determine the pH of common acids and bases using a pH meter‚ pH paper‚ and red cabbage indicator. To test the effect of adding an acid or base to a buffer solution. SAFETY CONCERNS: Always wear safety goggles. Wash with soap and water if skin contacts acids or bases. ACIDS: An Acid is a substance that when dissolved in water will produce hydrogen ions‚ H+‚ in the solution. An acid that does not contain carbon is called an inorganic
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Amylase Amylase Amylase Amylase 5 Deionized Water Deionized Water pH 2.0 Buffer Amylase 6 7 8 Reagent 2 Starch Starch Starch Deionized Water Starch Reagent 3 pH 7.0 Buffer pH 7.0 Buffer pH 7.0 Buffer pH 7.0 Buffer Treatment Boiled Frozen None None Time 60 60 60 60 Temp. 37 37 37 37 IKI + - Benedict’s ++ ++ - pH 7.0 Buffer None 60 37 + - Maltose pH 7.0 Buffer None 60 37 - ++ Starch Starch Amylase pH 9.0 Buffer None None 60 60 37 37 + + + + 04/08/15 page 2 Post-lab Quiz
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3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
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The globalization of markets Theodore Levitt The worldwide success of a growing list of products that have become household names is evidence that consumers the world over‚ despite deep-rooted cultural differences‚ are becoming more and more alike - or‚ as the author puts it‚ "homogenized." In consequence‚ he contends‚ the traditional MNC’s strategy of tailoring its products to the needs of multiple markets may put it at a severe disadvantage vis-a-vis competitors who apply marketing imagination
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Peel samples‚ coming from Ananas comosus‚ will undergo a series of physical and chemical analyses to determine the glucose content‚ which will be utilized to yield bioethanol. The SSF process will be manipulated in terms of fermentation time and buffer level used to determine the best variables for biofuel production. Introduction A. Background of the Study The concept of producing bioethanol from pineapple (Anae ananas comosus)
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Nick Sarris‚ April 3‚ 2013‚ D-Bell Biology Virtual Electrophoresis Lab – Genetic Science Learning Center Use the link to complete the following lab. Submit through edline when you are finished http://learn.genetics.utah.edu/units/biotech/gel/ Title‚ name‚ date and bell (8 pts) Place your answer below the question and skip between questions (2 pts) Each question is worth 3 points 1. Why can’t DNA be sorted physically‚ using a microscope?- They are so tiny that they are unable to be
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should : -Describe the problem -present an alternative if one is available -if possible ask for input of action The Parts of a Negative Message ■ Subject Lines ■ Buffers ■ Reasons ■ Refusals ■ Alternatives ■ Endings Subject Lines Buffers Traditionally‚ textbooks recommended that negative messages open with buffers. To be effective‚ a buffer must put the reader in a good frame of mind‚ not give the bad news but not imply a positive answer either‚ and provide a natural transition to the body of the letter
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In McCullen‚ the court states that the place the speech is given is the key detail. If a person is within the buffer zone then they are violating the act. Ms. Mendoza violated the Act when she entered the buffer zone and when she entered the floating buffer zone. The opposing would argue that the volunteers and people who work in the clinic does not have a content/viewpoint neutral view on this matter because they work at the
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tray to harden into a slab called a gel. A plastic comb inserted at one end while the gel is hardening forms wells where DNA samples can be placed. The DNA is mixed with a loading buffer that contains glycerol—this makes it heavier than water‚ so it will sink to the bottom of the well. The gel is then covered with a buffer solution that can carry electric current‚ and electrodes are placed at each end of the gel and connected to a power supply. Because DNA is negatively charged (each nucleotide has
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