contained a population of Gram negative bacteria. Each sample was placed in an HEPES buffer at a pH of 7.2 and concentration of 1.0 mM. This created a hypotonic solution. The first and second group contained three samples each. The first sample was a control and contained only the buffer and the Micrococcus. The second sample contained the buffer‚ the Micrococcus‚ and the lysozyme. The third sample contained the buffer‚ the Micrococcus‚ the lysozyme‚ and KCl used to create an isotonic
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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graph shows RFU’s (left) and total protein in ug (right) for each wash and elution. Once the rGFP had been purified in the Ni2+-agarose column‚ a breaking buffer (10mM Tris‚ pH 8.0; 150mM NaCl) was used to create the washes by pipetting the buffer in 0.5ml increments and collecting each in separate tubes labeling them W1-W6. Then‚ an elution buffer (10mM Tris‚ pH 8.0; 150mM NaCl; 300mM imidazole) was collected in increments of 0.5ml and labeled E1-E6. We could measure the amount of RFU’s each sample
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Our results indicated that at three different pH levels‚ the absorbance level of our samples was not the same for each one. This proved my original hypothesis to be incorrect‚ as I was surprised to find that the last pH buffer had no effect on the absorbance. The first two pH buffers supported my hypothesis‚ however. The levels of our samples kept decreasing over time. As with every experiment‚ it should be repeated several times to make sure your results are accurate. Introduction Most chemical
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Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish. Chae Yee Kent State University: BSCI 30140 Lab Abstract Western Blotting can be used to detect the Myosin actin light chain in different species of fish and is used to distinguish from different species based on variation‚ commonality‚ or evolutionary divergence. First‚ proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according
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leaders of the empire devised three ways to protect the empire from the barbarians. B. The leaders of the empire devised three ways to deal with the Huns and Mongols. III. The end of the empires A. Toward the end of the empire’s rule‚ some of the buffer states revolted brought on the empire’s breakdown. B. The barbarians broke through to the empire and set up dynasties imitating the Chinese way of life. Roman and Chinese Empires We know about some great
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Effects of Temperature‚ pH‚ Boiling‚ and Hydroxylamine on the Enzyme Peroxidase Extracted From Brassica rapa Abstract In this experiment the enzyme peroxidase was extracted from from a turnip‚ Brassica rapa‚ and tested under different conditions. The effects of temperature‚ boiling‚ pH‚ and a competitive inhibitor were tested. The enzyme was tested at temperatures of 4°C‚ 24°C‚ 32°C‚ and 48°C. As the temperature increased‚ so did the activity of the enzyme
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microorganisms for long-term storage. After use they should be washed for 30 minutes with 0.5M NaOH. Ni-NTA matrices should be stored in 30% ethanol to inhibit microbial growth. The matrix can be stored for up to one week in any of the denaturing buffers. Reuse of Ni-NTA Resin The reuse of Ni-NTA resin depends on the nature of the sample and should only be performed with identical recombinant proteins. Based on the experience of Hoffmann-La Roche Ltd. (Basel‚ Switzerland)‚ who have purified
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1.1 "Probabilitized" EOQ Model Some practitioners have sought to adapt the deterministic EOQ model to reflect the probabilistic nature of demand by using an approximation that superimposes a constant buffer stock on the inventory level throughout the entire planning horizon. The size of the buffer is determined such that the probability of running out of stock during lead time (the period between placing and receiving an order) does not exceed a prespecified value. Let L = Lead time between placing
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Examination of the Effects of Inhibitory and Non-Inhibitory Competition‚ Enzyme-Substrate Concentration‚ Along with Varying Temperature and pH-Balanced Environments on the Enzyme-Catalyzed Reaction of pNPP Abstract: Introduction: Many of the chemical reactions‚ which take place in in living things are controlled by enzymes. In such cases‚ the enzyme is a protein in the cell which lowers the activation energy of a catalyzed reaction‚ which serves to increase the rate of the reaction. Alkaline
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