ingredients found in an SDS-PAGE gel. Which ingredients are responsible for polymerizing the solution? How does the percentage of acrylamide effect the migration of proteins (ex: 4% gel vs. 18% gel)? 2. [8pts]Describe the purpose of each loading buffer ingredient added to protein samples for SDS-PAGE analysis (hint- there are 4 ingredients). 3. [45pts]You purified protein X via affinity chromatography (no diafiltration step performed) and ran an SDS-PAGE gel of the sample with a set of controls
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Optical Communication Concept R K Gangwar Divisional Engineer(Transmission) BSNL For circulation to Trainees only Agenda • History • Why Optical Fiber? • Applications of Optical Fiber • Transmission Sequence • Geometry of Fiber • Principle of Propagation of Light • Types of Optical Fiber For circulation to Trainees only History • • • • 1960: Laser invented 1967: New Communications medium: cladded fiber 1960s: Extremely lossy fiber: more than 1000 dB /km 1970‚ Corning Glass
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Bita Heydari Lab report 3 The Effects of Differentiation on Enzymatic Activity Introduction HL-60 cells are capable of undergoing differentiation to induce different cell types. HL-60 cells can undergo morphological changes‚ changes in gene expression‚ and changes in protein synthesis. In the past weeks‚ we were able to conclude that HL-60 cells treated with DMSO and HL-60 cells treated with PMA will differentiate into granulocytes and monocytes upon treatment (1). We were also able to observe
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Student Misconceptions • • • • • Students often believe that the pH at the equivalence point for any titration is 7.00. In terms of problem-solving skills‚ this is probably the most difficult chapter for most students. Students tend to find buffers particularly difficult to understand. Students often forget to consider volume changes that occur when two solutions are mixed (this will have an effect on the concentration of the species present). Students tend to confuse Ksp and solubility.
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The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
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Exam 3 Study Guide Chapter 11: Acid-Base Balance During Exercise Acids and Bases Acid Molecule that can liberate H+ (proton donor) Increases H+ concentration in solution Lactic acid is a strong acid Base Molecule that is capable of combining with H+ Bicarbonate (HCO3-) is a strong base pH Expression of H+ solution in solution Negative logarithm of H+ concentration pH = -log10[H+] pH of pure water pH (pure water) = -log10[H+] = 7.0 pH of Blood Normal pH = 7.4 ± 0.05 Acidosis pH
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Reactions of the DNA translocase FtsK and XerC & XerD recombinases from Pseudomonas aeruginosa Luhai Xu Student number 3107041 Supervisor Ian Grainge Table of Contents Abstract 3 LIST OF ABBREVIATIONS 4 Chapter1: Introduction 5 Chapter 2 Materials and Methods 26 Chapter 3: Results 41 Chapter 4: Discussion 63 References 74 Appendix 80 Abstract DNA replication is an important biological process and occurs in all living organisms
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Henry Su December 12‚ 2001 Chapter Notes Chemistry 221 Chapter 13 Dr. Rahni Titrating Polyfunctional Acids and Bases 13A—Polyfunctional Acids -Phosphoric Acid is a typical polyfunctional acid H3PO4 + H2O ( H2PO4- + H3O+ Ka1 = [H3O+] [H2PO4-] = 7.11e-3 [H3PO4 ] H2PO4- + H2O ( HPO4-2 + H3O+ Ka2 = [H3O+] [H2PO4-2] = 6.32e-8 [H2PO4- ] HPO4-2 + H2O ( PO4-3 + H3O+ Ka3 = [H3O+] [PO4-3] = 4.5e-13 [HPO4-2]
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Assignment 1: The goals of this assignment are: - learn to compile and run a very simple Java program - To know how to use environment (a) WAP to generate Fibonacci Series (b) WAP to generate Prime Number Series (c) WAP to convert given Celsius to Fahrenheit Assignment 2: The goals of this assignment are: - learn to compile and run a very simple Java program - learn to Arrays (a) WAP to find the sum of ‘n’ Numbers (b) WAP to Sort ‘n’ Numbers (c) WAP
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Characterization of a Yam Class IV Chitinase Produced by the Recombinant Pichia pastoris X-33 Department of Botany‚ Jahangirnagar University‚ Savar‚ Dhaka 1342‚ Bangladesh Abbreviations: Endo H‚ Endoglycosidase H; PR protein‚ pathogenesis related protein; P. pastoris‚ Pichia pastoris; VTS‚ vacuolar targeting signal; PCR‚ polymerase chain reaction; PAGE‚ polyacrylamide gel electrophoresis; SDS‚ sodium dodecyl sulfate Abstract A yam (Dioscorea opposita Thunb) class IV chitinase‚ whose genomic
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