methanol‚ methylene chloride and insoluble in water‚ ether and slightly soluble in PBS (pH 7.4)‚ dichloromethane and very slightly soluble in chloroform‚ alcohol etc. Partition coefficient of the drug was found to be 5.057 in n-octanol: Phosphate buffer solution (pH 7.4) suggesting the drug to be lipophilic in nature. Standard curves of Ketoconazole were prepared in different fluids i.e. PBS (pH 7.4) and methanol. The R2 values are 0.9987‚ 0.9983 and a straight line was obtained in both the cases
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Aim: The aim of this experiment is to investigate the process of Electrophoresis and successfully analyse DNA fragments. Hypothesis: That the experiment will show the visual representation of DNA proteins‚ and that the shorter the band the further is will travel. Background: Restriction enzymes are DNA-cutting enzymes found in bacteria‚ which cut DNA into smaller fragments. A restriction enzyme recognizes and cuts DNA only at a particular sequence of nucleotides‚ known as restriction sites
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The article examines the relationships between the black working poor and the black middle class. The black middle class is defined as being fragmented‚ comprised of an array of incomes‚ professions‚ and educational levels. The article also states that black middle class experiences economic shifts that move back and forth between blue-collar and white-collar income levels and occupations. (Shawn A. Ginwright 2002) The black middle class experienced dramatic growth in the post civil-rights era. This
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various fruits using cell disruption and separation techniques. Materials Used: 2 heavy duty zip-lock baggie 1 strawberry (fresh or frozen and thawed) 1 banana half 10 ml DNA extraction buffer* 2 Coffee filters Ice cold 95% ethanol 1 small beaker 2 Test tubes Wooden coffee stirrer *To make the extraction buffer‚ 100 ml of shampoo (without conditioner) was mixed with 15 g of NaCl and 900 ml of water. Procedures Used in Experiment Once all materials were gathered by group members‚ each individual
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Assignment of Biopharmaceutics I BCS Classification of Some Drugs Faculty- Dr. Rajib Bhattacharjee BY Shoaib –UL–Islam 0910783046 The Biopharmaceutical Classification System- The Biopharmaceutics Classification System is a guide for predicting the intestinal drug absorption provided by the U.S. Food and Drug Administration. It was developed primarily in the context of immediate release (IR) solid oral dosage forms. It is the scientific framework for classifying drug substances based
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1. A manufacturer’s average work-in-process inventory for a certain part is 1‚000 units. The workstation produces this part at the rate of 200 units per day. What is the average time a unit spends at this workstation? 2. The Wilcox Student Health Center has just implemented a new computer system and service process to \improve e±ciency." As pharmacy manager‚ you are con- cerned about waiting time and its potential impact on college students who \get no respect." All prescriptions (Rxs) go
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Effect of pH of the reaction: The impact of the pH of the reaction on the activity of the pure pectinase was studied. The pH range of 3.5to 8 was used (acetate buffer‚ 0.1M; phosphate buffer‚ 0.1M).all the other reaction conditions were at their optimum values as determined from the previous experiments. Table 27: effect of the reaction temperature on the activity of the purified pectinase obtained from Brevibacillus borstelensis
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affect project goals. In the triple-constraints (time‚ cost‚ scope)‚ time remained the most impacting constraint to projects. The critical chain resolved this issue by removing protection time from tasks‚ aggregating them into feeding and project buffers‚ and placing them in strategic locations within the project network where they will serve to protect the project as a whole. This way‚ the fixed elements of each task is the aggressive but possible estimate‚ whilst the variable components are called
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` PORTABLE MOBILE CHARGER USING PIEZOELECTRIC ENERGY DEEPAK SAHADEVAN NAIR PRADEEP KARUNAMBARAN SUBMITTED ON: 07/JUNE/2013 CIRCUIT DESIGN PROJECT REPORT 7514ENG PRACTICAL ELECTRONICS Griffith University‚ Brisbane‚ Qld‚ Australia EXECUTIVE SUMMARY Alternative energy sources are one of the main focuses in research and development in many aspects in engineering‚ especially electronic devices. By targeting renewable energy sources‚ not only will the amount of pollution decrease but also
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BIOL2220 Lab 3: Hill Reaction Introduction In 1937‚ Robert Hill discovered that isolated chloroplasts can generate oxygen when they are illuminated in the presence of a suitable electron acceptor‚ even in the absence of carbon dioxide. This finding was a landmark in the study of photosynthesis because it established that the source of the electrons used in the light reactions is water. It also confirmed that the released oxygen is derived from water instead of carbon dioxide. In chloroplasts
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