experiment: Effect of pH on enzymatic activity Background Information: Trypsin is a protease which conducts hydrolysis forming peptides. It is an enzyme which is secreted by the pancreas into the small intestine and works best in an alkaline environment. Egg white is used in this practical as it is found to contain the protein/ enzyme trypsin. pH is the measure of the amount of H+ ions in a solution‚ these ions affect the shape of the enzyme. Hypothesis: That as pH increases‚ the rate of
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Enzymes are naturally occurring biological catalysts that are extremely efficient and specific. Enzymes accelerate the rate of a reaction by factors of at least a million as compared to the same reaction without the enzyme. Most biological reaction rates are not perceivable in the absence of the enzyme. The term enzyme was first used by a German pshysiologist Wilhelm Kühne in 1897. There are over 700 different kinds of enzymes that have been identified. Enzymes can be classified into several categories
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A critical point happens when a substance has a high enough temperature and pressure that both liquid and gas can exist at the same time. This state is called a critical point. Now‚ when a state of matter changes from one state to another is called a phase shift. Phase shifts are extremely important and most commonly observe phase shifts daily. For an example‚ when water freezes into ice‚ or the water in the shower turns to steam. To begin to start describing how a phase shift works we must understand
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…………………KEY……………………… Matter & Energy Period …………. Skills 1. classifying types of matter 2. interpreting particle diagrams 3. identifying physical & chemical properties of matter 4. separating Mixtures 5. converting Temperatures 6. identifying physical & chemical changes in matter Skill #1: Classifying types of matter - refer to your notes and RB p. 1-2 Classify each of the following with the combination of terms listed below. pure substance – element mixture
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‘Kinetics’ is the study of chemical reactions. The speed of a reaction can vary depending on many variables such as the nature of the reactants‚ particle size‚ concentration and temperature. For a chemical reaction to occur‚ there must be a collision between reactants. The reaction rate is slower when the reactants are large and complex molecules because it takes longer for the molecules to combine together creating a chemical reaction. More than 2000 years ago‚ Democritus‚ who was a philosopher
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Research Question: To investigate how varying the pH of bromothymol blue affects the absorbance value of the solution which determines the equilibrium constant (pKa) of the indicator. Variables: Variables Variables Measured Method of measurement Independent pH of the six buffer solution A pH probe attached to a data-logger will be used to measure pH Dependent Absorption of the buffer solutions at wavelength 435.0nm and 617.0 nm A spectrophotometer (±0.001) will be used to measure absorbance
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How Enzyme Concentration can Affect Rate of Reaction The purpose of this investigation was to see how the concentration of an enzyme affected the rate at which a substance was broken down. We did this by using a white protein called casein. Casein is found in milk powder‚ it is a protein and used mainly as a binding agent in foods‚ because it is mad to proteins and joins to a phosphoric acid it belong to a group called the phophoproteins. In terms of in milk it is said to be healthier if it is
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The pH Scale is a scale made to show how acidic or alkaline a substance is. The pH of something is measured with pH paper which can vary from wide range paper‚ which covers all ph’s‚ but is not as accurate at some others. There are pH papers that get very specific that can have a range such as 2 or 3 and get much more precise. The neutral on the scale is 7. Anything that is below 7 is considered acidic and anything above 7 is considered alkaline. Acidic substances contain more H+ than OH-. H+
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Introduction Enzymes are proteins produced by living organisms to speed up the rate in which chemical reactions occur. This process can happen fast‚ slowly‚ or stop the chemical reaction all together depending on the temperature‚ pH and concentration. Catalase is one of the most common enzymes. It is found in living organisms and is used to break down hydrogen peroxide. This must happen because hydrogen peroxide is considered toxic to cells in the body. However‚ when catalase is used it breaks
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The affect of temperature on the rate of movement of woodlice Aim: The aim of this experiment is to establish whether a change in temperature affects the rate in which woodlouse move. I measured the rate by timing the woodlouse to move a set distance of 20cm‚ and altered the surrounding temperature by submerging a clear tube in water with different temperatures controlled by a water bath. Research: It is to be believed there over 3000 different species of woodlice‚ a total of 42 species
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