FUNCTIONS OF ENZYMES Enzymes are extremely important to the human body and its ability to function. An enzyme itself is a protein made by the body’s cells to act as catalyst‚ speeding up chemical reactions in the cell. It does this by taking the reactants‚ or the elements or compounds that enter into the chemical reaction‚ in this situation referred to as substrates‚ and breaking apart their bonds so that new ones can form. The three types of enzymes are digestive enzymes‚ food enzymes‚ and metabolic
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Immobilized Enzyme Immobilized Enzyme * Enzymes which are attached to small bead made of alginate are called immobilized enzyme * However‚ whatever the nature of an immobilized enzyme‚ it must comprise two essential functions‚ namely the non-catalytic functions (NCF) that are designed to aid separation and the catalytic functions (CF) that are designed to convert the target compounds within the time and space desired * Immobilized enzymes might meet the increasing demand by manufacturers
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Lab 6: Enzymes . header Purpose: The purpose of this lab is to test for enzyme activity‚ look at enzyme specificity‚ and how temperature affects enzyme activity. Time need to perform this lab: approximately 3 hours Preparation FIRST: Read the lab in its entirety TWICE before you begin. You will perform the experiment‚ write your lab report and include the answers to the additional 4 questions within the text for full credit on this experiment. Materials: •3% hydrogen peroxide •a
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substrate concentration‚ the enzyme is working at “maximum efficiency.” With a concentration at 40‚ it produced 2‚339 products. 2. The maximum velocity of a reaction is reached when the active sites are almost continuously filled. Increased substrate concentration after this point will not increase the rate. The reaction rate increases as substrate concentration is increased. It will soon level off though. 3. When the concentration is at low substrate‚ most of the enzyme molecules are not filled
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BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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Abstract This experiment is to study and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient
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Group No. Date: Leader : Section: Members: Experiment no: 1A Data and Results: Initial Temperature: To = 25 degrees Celsius Final Temperature: Tf = 100 degrees Celsius Rod | Initial length | Change in Length | Coefficient of Linear Expansion(Exp) | Coefficient of Linear Expansion(Theo) | % Error | Steel | 40 | .035 | 1.17 x 10 / C | 1.1 x 10^-5/ C | 6.36% | Copper | 40 | .055 | 1.8 x 10 ^-5 /C | 1.7 x 10^-5 /C | 5.88% | Computation : Conclusion: 1. A bemetalli
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Abstract This experiment was designed to answer the question does temperature affect the amount of osmosis? The hypothesis predicted was that the higher the temperature the more osmosis would occur‚ but too high the osmosis would halt due to enzyme and substrate overheating and losing shape. After research and class time it was concluded that osmosis is a passive transport and would not require energy or enzymes due to it going from high to low concentrations with the gradient
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Science investigation Aim: To try to see if the temperature affects the rate in which Amylase breaks down starch into maltose. In this reaction starch is the substrate and maltose is the product. Amylase is an enzyme‚ Enzymes‚ also called catalysts‚ are in living things and there are thousand of them. Enzymes break down food by the active site on the Enzyme forming a chemical bond with a substrate and then water attacks the substrate until it is hydrolysed (split in 2). Equipment: Boiling
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The purpose of this experiment is to study how enzyme activity is affected by environmental conditions. Researchers tested the level of potato extract enzyme activity with 1-11 pH‚ varying temperature‚ catechol solution‚ hydroquinone solution‚ and different measurements of catechol. In Figure 1A and 1B‚ pH levels were tested with potato extract to see how pH would affect the amount of Benzoquinone is formed in the potato. Although it was hypothesized that enzymes would form Benzoquinone better in
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