Ph Lab Report Bryon Kim 123013 B(2) Biology Background information/Research PH paper (litmus paper) determines how acidic or how basic a substance is. The paper changes color accordingly to color code on the pH scale. The pH scale starts from 0 to 14. The lower the number the more acidic it is. Zero is the most acidic‚ and 14 is the most basic while 7 is the neutral number for example water. Examples of an acid is lemon juice or multi purpose cleaner. Examples of a basic substance is shampoo or liquid soap
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Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature of the
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for the pH concentration experiment were put together by using a 10ml-graduated cylinder to obtain 4ml of each pH buffer to insert into cuvettes‚ a micropipette was then used to obtain 0.5ml of catechol and 0.5ml of the catechol oxidase. The pH buffer was made first to avoid any denaturation of the catechol oxidase. Our positive control for this experiment was pH 7 because that is the pH level of most cell membranes in the cytoplasm (Whitson‚ 2016.) Our negative controls varied for each pH buffer
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How does water temperature affect the amount of dissolved oxygen? Ⅰ. Introduction Dissolved oxygen is just one of the many ways to measure water quality. It means the volume of oxygen that is contained in water. The amount of dissolved oxygen in water usually determines the quantity of organisms living in that water. Dissolved oxygen levels vary by how much oxygen the water can hold‚ how much oxygen is produced by photosynthesis‚ and how much oxygen is consumed by respiration. If water is too warm
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Investigating the effects of changing temperature on the activity of enzymes Background information: Renin is an enzyme that catalyses the coagulation of milk. It is found in the stomach of many animals and is used in making cheeses and junkets. It is found in the gastric juices or gastric mucosa of many mammals‚ including humans. In the human stomach‚ particularly those of infants‚ rennin works to curdle milk so that pepsin‚ another stomach enzyme‚ can further breakdown the proteins into absorbable
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How Does the Water Temperature of the Frog’s Environment Affect Its Physiological Factors? Defining the Problem and Selecting Variables In the investigation of "how does the water temperature of the frog’s environment affect its physiological factors"‚ the dependent variable is the frog’s physiological factors‚ the independent variable is the water temperature of the frog’s environment‚ and the relevant controlled variable is the frog’s body temperature‚ heart rate‚ breathing rate‚ and blood pressure
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Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
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The Activity of a Protease (Trypsin) Introduction Enzymes catalyze reactions by creating alternate reaction mechanisms whose transition states are more thermodynamically stable than uncatalyzed reactions (Berg et al.‚ 2002; UBC Department of Microbiology and Immunology‚ 2006). Increased thermodynamic stability in these transition states reduces the energy of activation‚ the minimum amount of energy input a chemical system requires for a reaction to occur (UBC Department of Microbiology
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AUTOMATIC TEMPERATURE CONTROLLED FAN H ere is a circuit through which the speed of a fan can be linearly controlled automatically‚ depending on the room temperature. The circuit is highly efficient as it uses thyristors for power control. Alternatively‚ the same circuit can be used for automatic temperature controlled AC power control. In this circuit‚ the temperature sensor used is an NTC thermistor‚ i.e. one having a negative temperature coefficient. The value of thermistor resistance at
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Enzyme Lab Using Jello INTRODUCTION: Enzymes are known as protein catalysts. The name protein catalyst suggests that most enzymes are made of proteins. A catalyst is a substance that speeds up chemical reactions without being consumed in the process. (Giuseppe‚ M 2002‚ p.69). After a reaction has been catalyzed‚ the catalyst can be used again to catalyze the same reaction. Enzymes reduce the activation energy (minimal energy) it takes for a reaction to take place. Enzymes can either catabolize
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