Title: Enzyme Activity Aim: To investigate the activity of the enzyme catalase in liver and potatoes‚ and to investigate the effect of temperature‚ surface area‚ pH and certain chemicals on the activity of catalase. Equipment: Dilute hydrochloric acid solution x 1ml 10 volume hydrogen peroxide x 100ml Copper sulphate solution x 1ml Aluminum nitrate solution x 1ml Zinc nitrate solution x 1ml dilute NaOH solution x 1ml Mortar and pestle x 1 25ml Beaker x 1 Hot plate x1
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details. By observing the responses to these tests performed in a particular sequence‚ some can be eliminated as possibilities and others require further investigation. Materials and Methods: A stock culture‚ labelled 25‚ was stored at room temperature (in the lab desk). A transfer loop was sterilized and a sample of the stock was transmitted to a tube of sterile BHI broth (for a working culture) and also to an agar plate and incubated for 24 hours. From the incubated plate‚ distinct and well
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"To See What Factors Affect The Decomposition Of Hydrogen Peroxide By The Enzyme Catalase Which Is Found In The Liver" Introduction: Enzymes are biological catalysts. They speed up the chemical reactions which go on inside living things. Without them the reactions would be so slow that life would grind to a halt. Enzymes work by when a substrate molecule bumps into a molecule of the right enzyme‚ it fits into a depression on the surface of the enzyme molecule. This depression is called the
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conclusion that my bacteria was a positive cocci. After the gram stain I performed specific tests based of those results of the stain to determine what my unknown could be. The first test I did was the catalase test. It had a positive reaction meaning the bacteria produced the enzyme catalase. This helped me narrow my unknown down to Micrococcus and Staphylococcus. I inoculated my unknown onto a mannitol salt agar. The growth and color change to yellow indicated mannitol fermentation because acid
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LAB: Substrate Concentration Affecting the Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because
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the two combine at an area called an active site. This active site allows induced fit which allows the reaction to occur and a new product to be formed. In the experiment we utilized the equation: 2 H2O2(aq)-> 2H2O(l) + O2(g). Through the use of a catalase such as the potato‚ hydrogen peroxide is broken down to release water and oxygen. Mixtures were allotted time to react‚ with those have a longer time resulting in more substrate being able to be acted upon in correlation. The sulfuric acid was added
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Oxygen Requirements of Bacteria BACKGROUND The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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How is pH affecting catalase activity? Hypothesis The catalase activity is assumed to be the most active in pH7. The higher or lower concentration away from the optimum pH of catalase‚ the slower the rate of activity is. Data Collection & Processing I collected the results of pH4‚ pH7‚ pH10 and pH13 after 2 minutes‚ and I repeated the experiment 3 times. Below is a table to show the results‚ their averages and standard deviations: | 1st time (sec) | 2nd time (sec) | 3rd time (sec)
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Title: The effect of the concentration of hydrogen peroxide on catalase Aim: to examine how the concentration of the hydrogen peroxide affects the rate of reaction Hypothesis: The substrate concentration will determine the rate of reaction. The reaction will increase as the concentration increases‚ but from a certain concentration‚ the rate will maintain the same rate Materials: 3% hydrogen peroxide 6% hydrogen peroxide catalase round metal basin measuring cylinder thermometer conical flask
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