Investigate Concentration of Substrate on Catalase enzyme 1. Aim/Prediction. Aim of this experiment is to investigate concentration of substrate on catalase enzyme‚ as well to see how they work in different concentrations of hydrogen peroxide (H2O2). Prediction would be‚ that the filter paper discs that were used‚ will rise up to the surface in a time in the tube of different concentration of hydrogen peroxide. And in different concentrations the time will be different. As higher the concentration
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Aim: Investigate the effect of different temperatures and chemicals including various pH levels‚ alcohol and detergent on the cell membrane structure as well as function. Hypothesis: Increasing the temperature and pH levels which the beetroot membrane cells are exposed to will increase the amount of dye that passes out of the membrane. Materials: Part A • Bunsen Burner‚ Tripod and a Wire Gauze • 250mL Beaker • A 100° thermometer • Matches • 10mL measuring cylinder • Sticky labels and a Pen
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available to react and the O2 is produced at lower rates. When no more peroxide is left‚ O2 is no longer produced. Objectives Use a computer and an Oxygen Gas Sensor to measure the production of oxygen gas as hydrogen peroxide is destroyed by the enzyme catalase at various enzyme concentrations. Measure and compare the initial rates of reaction for this enzyme when different concentrations of enzyme react with H2O2. hypothesis Write a 2-part hypothesis that predicts what you think will happen in this investigation
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test tube 3 6 2.6 Errors 6 3. Conclusion and Evaluation 6 3.1. Conclusion 6 3.2. Evaluation 7 3.2.1. Random Errors 7 3.2.2. Systematic Errors 7 3.3. Improvements 7 1 Design Look to sheet titled: ’Investigating the action of the enzyme catalase’ 1 1.1 Variables The independent variables are the acids used‚ the dependant variables the height of the bubbles formed and the control variables the test
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The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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Date: Wednesday‚ November 16‚ 2011 Holly Lawson - white Title: API 20E Introduction: The general principle of the experiment is to use a battery of biochemical tests for identification of enteric bacteria. This testing system consists of a strip containing 20 chambers‚ each consisting of a microtube and a cupule to allow testing of 20 different tests nearly simultaneously. Materials: inoculating loop‚ test tube containing 5 ml of sterile saline‚ wash bottle of water‚ plastic API
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large molecules and detoxify hazardous substances. It contains enzymes like oxidase‚ which can create hydrogen peroxide as a byproduct of its enzymatic reactions. Within the peroxisome‚ hydrogen peroxide can then be converted to water by enzymes like catalase and peroxidase. Glyoxysomes Glyoxysomes are specialized peroxisomes found in plants and mold‚ which help to convert stored lipids into carbohydrates so they can be used for plant growth. In glyoxysomes the fatty acids are hydrolyzed to acetyl-CoA
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Environmental Variables and their effect on Horseradish Peroxidase Abstract There are several factors that can affect the rate of reaction of peroxidase such as temperature‚ pH‚ concentration of peroxidase present and whether or not it has been boiled. Our experimental data demonstrated that peroxidase activity peaked between 23 degrees Celsius and 32 degrees Celsius. We found the pH to be 7 for optimal activity. As far as the concentration is concerned our results showed that as the concentration
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Investigating the effect of changing substrate concentration on the activity of the enzyme catalase The aim of this experiment is to examine how the concentration of a substrate (hydrogen peroxide) affects the rate of reaction of an enzyme catalyse (found in liver cells) Research Question: how does changing the concentration of the substrate affect the rate of reaction of the enzyme catalyse? Hypothesis: As the concentration of the substrate increases‚ so does the rate of reaction until the reaction
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From the graphs‚ it is evident that an increase in both catalase concentration and substrate concentration resulted in a higher rate of reaction or‚ as observed in the kPa graphs‚ a higher volume of O2(g) formed at the end of the 5 minute trial. Interestingly‚ it should also be noted‚ as it was mentioned in the Figure 2‚ that the trend for the 6mL of 3% H2O2(aq) was more of a linear trend than an exponential decay‚ steadily rising until the end of the 5 minute trial. From this‚ it can be inferred
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