Title : THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim : To study the effect of pH on the activity of catalase. Introduction : Catalase‚ an enzyme found in many different tissues‚ catalyses the breakdown of hydrogen peroxide into water and oxygen. 2 H202 → 2H20 + O2 Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen peroxide
Premium PH Acid Enzyme
Plants in Temperature An Investigatory Project Presented To the High School Department of the New Jerusalem School In Partial Fulfilment of the Requirements in
Premium Water
Temperature Rising Global Warming is the increase in the average temperature of the Earth’s near-surface air‚ landmasses‚ and oceans its projected prolongation. Many believe that the Earth is currently facing warming in the ozone layer brought on by the rising levels of trapped gases known as the greenhouse effect resulting especially from pollution. Global warming is‚ in Laymen’s terms‚ altering the balance in energy of the earth (Reid 27). “The topic of climatic change has been in question
Premium Greenhouse gas Carbon dioxide Global warming
the activity of the enzyme (catalase)? Theory The higher the substrate concentration the more quickly product is produced (rate of reaction increases) until enzyme saturation is reached at which time more substrate has no further effect. Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all very specific as each enzyme just performs one particular reaction. Catalase is an enzyme found in food
Free Enzyme Hydrogen peroxide Oxygen
INTRODUCTION The enzyme catalase converts hydrogen peroxide (H2O2) to water and O2. The evolution of O2 causes bubbling. Thus‚ catalase-positive organisms that are mixed into hydrogen peroxide will cause bubbling (catalase-negative organisms will not). This test is good for distinguishing between Gram-positive cocci in chains (catalase negative) versus Gram-positive cocci in clusters (catalase positive). The coagulase test is used to differentiate Staphylococcus aureus from coagulase-negative
Premium Bacteria Staphylococcus Staphylococcus aureus
Obtain three test tubes and label each with the numbers 1‚ 2‚ and 3 with a glass-marking pen (preferably on a piece of masking tape) and place them in a test tube rack. Add a 0.5 cm cube of Beef liver into each tube (if not already prepared‚ use a scalpel or knife to cut your own pieces). Using a medicine dropper‚ add 3-4 drops of pH solution 2 for test tube 1‚ pH 7 for test tube 2‚ and pH 11 for test tube 3. Add a few drops of tap water for each tube. Then‚ add 2-3 drops of hydrogen peroxide
Premium Enzyme Chemistry Chemical reaction
the rate of catalase activity The extent at which environmental factors affect the rate of catalase activity was discovered in this lab. The assay system‚ in which a filter paper disc was dipped into the enzyme and submerged using a stirring rod in a test tube filled with 20mL of hydrogen peroxide‚ was used to test several enzyme factors. As the saturation of hydrogen peroxide increased the rate of reaction increased as well. When the enzyme concentration increased the rate of catalase activity
Premium Management Strategic management Computer
this unknown sample. First‚ the plate was analyzed for its hemolysis characteristics. Afterwards‚ a Gram stain was performed from a sample of the agar plate and the slide was viewed under the microscope. Once‚ the microscopic visual was captured‚ a catalase test followed. Next‚ for further data‚ MSA results were recorded‚ along with antibiotic observations of demo plates. Lastly‚ with the information gathered‚ it was identified that the unknown sample was Streptococcus salivarius. Introduction:
Premium Bacteria Microbiology Staining
30%‚ the change in temperature was very quick but plateaued at 25 degrees just as quick. For the 20%‚ the change in temperature was completed by around the 3rd-minute mark‚ so relatively fast before plateauing at 25 degrees. But the 3% took almost the whole 7 minutes to finish rising in temperature. So essentially‚ the higher the concentration of hydrogen peroxide‚ the faster the reaction will take place. Furthermore‚ the foam (water and oxygen produced by the potatoes catalase reacting with hydrogen
Premium Chemical reaction Oxygen Enzyme
Enzymes are biological catalysts that speed up chemical reactions by lowering the amount of activation energy needed to start the reaction and let the reaction occur at temperatures found in living cells. The way that enzymes do this is explained with the lock and key hypothesis. This hypothesis says enzymes have a specific shape called the active site which is different between different enzymes. Molecules called the substrate that participates in the reaction also have a specific shape that can
Premium Enzyme Chemical reaction Catalysis