Question: Effects of pH on amylase activity Introduction: Amylase is an enzyme that is in human’s saliva as well as the pancreas. Enzymes are biological catalysts that speed up a chemical reaction. They break down complex molecules into simple ones. In this case‚ amylase converts starches (complex molecule) into simple sugars. That is why foods like potatoes for example‚ may taste sweet to us‚ because they contain starch. The optimum pH for pancreatic amylase is the pH of 7. In the experiment
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Effects of pH on fungal amylase activity BI 211 November 25‚ 2011 Introduction In recent years‚ the uses of microorganisms have become a huge importance to industry and sparked a large interest into the exploration of enzyme activity in microorganisms. Amylase is one of the most widely used enzyme required for the preparation of fermented foods. Apart from food and starch industries‚ in which demand for them is increasing continuously‚ amylase is also used in various
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Temperature and Thermometers The Temperature of an object is a measure of the hotness or coldness of that object. An alternative way to think of temperature is to say that “the temperature of an object is a number – on some manmade scale – that indicates the hotness of the object”. ‘Hotness’ in turn is a measure of the kinetic energy of the molecules of the material. Note: You must use the term ‘hotness’.* The SI unit of temperature is the Kelvin (K)* Relationship between degrees Celsius
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Abstract: This experimentation was to evaluate absorbance and the reaction rate of an enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To
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What amount of amylase is the best to produce a better reaction with starch? A 1% solution of amylase‚ made from 0.15g of amylase added to 15ml of water‚ a 2% solution of amylase made of 0.3g of amylase added to 15ml of water‚ and a 0.5% solution of amylase made of 0.75g of amylase to 15ml of water were created. A 0.25% starch solution was also created‚ and 3ml was added to each of the 4 test tubes. 1 drop of iodine was added to each test tube. 4ml of the 1% solution was added to the first test
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What effect does temperature have on the oxygen production of enzymes in an animal’s liver? A temperature which is too high would be able to produce little to no oxygen because of the extreme temperature. Just like a temperature too high‚ a temperature which is too low would also produce little to no oxygen for the same reason. Animals that have livers are considered warm-blooded‚ with very warm temperatures; therefore it would make sense for a warm temperature to produce the most oxygen; but one
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pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities‚ one of the enzyme that was used in the process was amylase. When a serial dilution was
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Experiment 4 – Effect of Temperature on Enzyme Activity Aim To study the effects of temperature on the activity of amylase enzyme on starch solution. Introduction Enzymes are widely known as biological catalyst. Almost all cellular reactions are controlled and guarded by enzymes. Virtually every metabolic reaction which takes place within a living organisms are catalyzed by enzymes. Enzymes are complex three-dimensional globular proteins. Some of the enzymes are built up off proteins and
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Investigation Into Temperature Sensors Introduction In this project I will be investigating how it is possible to use a temperature sensor to keep a greenhouse from changing temperature too much. This is intended to help plants live and grow in their optimum temperature. This will create a perfect temperature for the enzymes in the plants to work in‚ and therefore resulting in a maximum growth/production rate. This could be useful for gardeners who wish to grow plants as quickly as possible
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Heat and Temperature Heat is often described by the average individual as being the change in temperature from hot to cold. “Often the concepts of heat and temperature are thought to be the same‚ but they are not.” Heat and Temperature‚ para. 2) Perhaps the reasoning behind the incorrect reasoning is that humans associate the two together because when heat is applied to an object the temperature rises. The kinetic theory of matter better explains the underlying cause as to what takes place
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