Enzyme Lab Experiments Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme? Materials: G;lucose Test Strips Test Tubes Pipettes Raw Hamburg Lettuce Potato Raw Liver Chalk Beakers Dairy Lactose Tablet Water Sugar Solo Cups Hot Plate Knife Gloves Skim Milk Glow Sticks Peroxide Hypothesis: 1. If we change the environment via temperature the glow stick will Its intensity will change 2. If hydrogen peroxide is added to a certain food liver
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The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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In fermentation the pyruvate is converted into ethyl alcohol‚ through the oxidation of the 2 NADH molecules‚ which returns them to two NAD+’s (Freeman‚ 2011). Oxidation is the loss of an electron in this case H+. We used information from previous labs in which we tested
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The normal breathing test tube took the longest then after hyperventilation‚ and then after exercise had the biggest CO2 concentration because of how quickly it was detected. See Table 1. In the second experiment‚ the original pH of the alkaline phenol red was 4.0. After
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Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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the dialysis tubing to glucose‚ starch‚ and iodine. Initially‚ glucose and starch were placed into the bag‚ and iodine outside of the bag in the beaker. We used the iodine test for the presence of starch which proved positive inside of the bag‚ and Benedict’s reagent for glucose which was positive outside of the bag. We therefore concluded that only small molecules like glucose and iodine can permeate through the bag‚ starch is too large. Introduction This lab has several key objectives. First
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Determining the Catalytic Properties of the Enzyme Peroxidase Extracted from a Turnip Under the Conditions of Temperature‚ pH‚ Boiling and Competitive Inhibitors By Robin Caserta BIO 101 September 30‚ 2013 ABSTRACT The enzyme‚ peroxidase‚ extracted from a turnip was tested for its efficiency in binding to its substrate and its stability under several conditions. To do this‚ we tested effects on peroxidase activity‚ first‚ with different amounts of the enzyme‚ next at temperatures of
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Potato Osmosis and Diffusion Lab Report Emily Cocq Research Question: Will there be a difference in the experimented potato cores after being subjected to high concentrations of sugar? Introduction: Water can move through protein channels in cell membrane/cell walls. The water will move due to the water potential of the cells. If there is a greater concentration of solutes (chemicals) inside the cell than outside the cell and the chemicals can not move‚ then water will respond by moving
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Created By: Andrew Weeldreyer Mrs. Wolf Period:3 AP Chem Lab Report: Determination of the Molar Volume of a Gas Objective: To react Mg and HCL and form hydrogen gas‚ then after collecting lab data‚ determine the hydrogen gas’s molar volume at STP through calculation. Materials: -LabQuest data collector - 3.0 M of HCl solution - Ribbon of Mg metal - Gas pressure sensor
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Alka-Seltzer Rocket Formal Lab Report Abstract In the Alka-Seltzer Rocket lab one was to experimentally determine how much (grams) Alka-Seltzer and water in mL‚ is needed to launch the rocket the highest/longest distance in cm. Also to experimentally determine the % NaHCO3 in the tablet using the information from the highest/longest distance. Calculate the error and % error of NaHCO3 in an Alka-Seltzer tablet and to determine the limiting reagent for the highest/longest distance traveled. Alka-Seltzer
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