techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate reduction‚ etc. Biochemical testing is very important in
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Purpose: To use a set of standardized procedures to test for simple sugars and starch‚ proteins‚ and fats. Hypotheses: A.) B.) C.) Equipment: -Safety goggles -Lab apron -400 mL beaker -Utility stand with ring clamp -Hot plate -Thermometer -10 mL graduated cylinder -Test-tube racks -8 to 16 test tubes -Test tube holder -Medicine dropper -Rubber stoppers -Test-tube brush -Wax pencil -Distilled water
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Amylase Enzyme vs. Starch vs. pH vs. Temperature Taylor Ellsworth Professor Michael Bunch Cell Biology 112 “Effects of Amylase reaction time when breaking down starch.” Experiment Goal: The goal of our experiment was to understand the similarities in digestion by finding out how long it takes for the amylase enzyme‚ found in saliva‚ to break down our substrate‚ starch. Hypothesis: While understanding that starch is broken down by our saliva (amylase enzyme) we predict that the higher
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The presence of carbohydrates in a solution can be determined by a qualitative test of Molisch test. Molisch test is a general‚ sensitive chemical test and positive for all kinds of carbohydrates which in free form or in combined form. The test is based on the dehydration of the carbohydrate by concentrated sulfuric acid to produce an aldehyde which condenses with two molecules of phenol‚ resulting in a coloured compound. Molisch reagent is a solution of α-napthol in 95% ethanol. The aldehydes produced
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My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
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non-reducing sugars‚ starch‚ lipids and proteins in unknown substances: Our aim: was to find out which substances from our five samples have reducing sugars present to determine what they may be for example; they could be monosacchrides or disaccharides. This was carried out by using Benedict’s test. To find out which substances from our five samples were non-reducing sugars‚ since some disaccharides are reducing sugars and this would be carried out by using Benedict’s test. To find out which
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least difficult sort of starch. Sugars are a wellspring of vitality for your phone’s‚ frame a portion of bigger‚ more perplexing particles‚ and give vitality stockpiling. Since your body rapidly processes sugars‚ they additionally give you a fast wellspring of vitality. There are three sorts of sugars. The three different types of sugars are monosaccharides‚ disaccharides‚ and polysaccharides. Monosaccharides otherwise called basic sugars‚ is the least difficult starch structure. When all is said
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Comparison of Extended Column Test (ECT) and Propagation Saw Test (PST) Introduction: Stability or “Instability” Tests are an important tool for determining the likelihood of a slope to produce an avalanche. Many different tests have been developed over the years – each with their own strengths and weaknesses. These tests provide a way to safely apply stress and deformation to a slope that is steep enough to slide (usually 250 or steeper) and observe and quantify the results. These results
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imply the non-stationarity of the linear combinations of these series. Hence‚ it is a prerequisite to ensure that all variables are integrated of order one in levels before employing the panel cointegration tests. Table 3 presents the results performed by Im‚ Pesaran and Shin unit root test of all variables for the four panels of economies tested in both levels and in first difference. Panel A‚ B‚ C and D represents all income economies‚ high-income economies‚ upper-middle-income economies and
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1. tested and measured? The purpose of this experiment was to determine whether the dark or light coleus leaf had the presence of starch. We used a dark and light leaf to test the presence of starch and how much was produced. 2. Summarize the results and how the results were obtained. Be sure to note the reason for each treatment of the leaves. As appropriate‚ compare experimental samples to controls. Both leaves were boiled in water then placed in hot ethyl alcohol. After adding the iodine
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