Amylase Enzyme vs. Starch vs. pH vs. Temperature Taylor Ellsworth Professor Michael Bunch Cell Biology 112 “Effects of Amylase reaction time when breaking down starch.” Experiment Goal: The goal of our experiment was to understand the similarities in digestion by finding out how long it takes for the amylase enzyme‚ found in saliva‚ to break down our substrate‚ starch. Hypothesis: While understanding that starch is broken down by our saliva (amylase enzyme) we predict that the higher
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experiment analyzes the effects of how temperature affects bacterial and fungus amylase and also discovers the optimum temperature for these enzymes. The amylase was mixed with starch at temperatures of 0℃‚ 37℃‚ 57℃‚ and 90℃. Iodine was added to each mixture and colour changes in each case. Bacteria amylase was found to be effective at 55 0C as the temperature dropped drastically from 4.58℃ to 2.33℃. This shows that the amylase catabolized a lot of starch hence little is left which cannot turn the iodine
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The Effect of Temperature on Animal and Fungal Amylase’s Ability to Breakdown Starch. Abstract This experiment was designed to test the reaction of the enzyme amylase at various temperatures. There were two different kinds of amylase being tested‚ one was fungal amylase also known as aspergillus oryzae and human amylase. The changes in temperature effect the rate at which an enzyme and a substrate collide. When the temperature is too high the active site changes shape or denatures‚ once this
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salivate is because your body wants to release an enzyme called amylase. Amylase is the enzyme found in your saliva. The purpose of this enzyme is to help in the digestion process (McD‚ 2002). Enzymes are biological catalyst that help speed up the reaction by lowering the activation energy. These enzymes allow your food to be digested at a faster rate (National Institute of Asian Medicine‚ 2007). There are many factors that can affect the efficiency of enzymes which include pH levels‚ the amount of
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Purpose The purpose of the lab is to determine how different factors affect the digestion of starch. Starch is a type of complex carbohydrate with large polysaccharide molecules that are made up of hundreds of glucose subunits. The digestion of starch begins in the mouth with the enzyme amylase and continues in the small intestine. Maltose‚ a disaccharide‚ breaks down the large polysaccharide molecules. Maltase‚ also found in the small intestine‚ splits each maltose molecule
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build and nourish cells; and to provide energy. Food travels though the digestive tract by muscle contraction called peristalsis. This journey of digestion can take up to twenty four hours. Digestion begins in the mouth where the teeth break food down into smaller particles during mastication. Salivary glands secrete saliva which begins chemical digestion and keeps the food moist. Swallowed food is pushed into the pharynx by the contraction of multiple muscle of the face‚ neck and tongue. The soft
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Identification of unknown a-Amylase through testing different temperatures and pH values to detect the absorbance of maltose. Introduction: Enzymes are biological catalysts‚ mainly proteins for this experiment‚ generated by an organism to speed up chemical reactions. They have active sites on which the substrate is attached‚ and then broken up or joined. For this experiment we are going to work with the enzyme a-amylase. Amylase is an enzyme that breaks starch down into sugar. Amylase is present in human
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Abstract This lab was focused on determining the optimal temperature of the enzyme amylase responsible for catabolizing starch polymers and to see how different temperatures affected the rate as well as how effectively the enzyme worked. To proceed with the experiment the group set up four different test tubes for each‚ bacteria and fungal amylase‚ and labeled them accordingly with different temperatures as well as different solutions . Then the spot plates were placed on the time and temperature
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PH level of Amylase Background info: What is Amylase? Amylase is an enzyme that helps digest carbohydrates. It is produced in the pancreas and the salivary glands. (Dugdale & Longstreth‚ 2011) Factors Affecting Amylase: Things that affect the efficiency of Amylase are temperature and pH levels. (Wikimedia Foundation‚ Inc‚ 2013) Function in the body: The function of Amylase in the human body is to break down plant-based starch sources. Therefore‚ providing the human body with more
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Chapter 10 Digestion and Nutrition Multiple Choice Questions 1. Plankton is a. Any organic debris in the ocean B. Plant and animal microorganisms drifting in the ocean c. A group of filter-feeding microorganisms d. Oceanic bacteria e. Seaweed 2. Examples of a suspension feeder and a deposit feeder would be‚ respectively‚ a. Clams and fish B. Polychaete annelid and earthworms c. Earthworms and rotifers d. Basking sharks and rotifers e. Earthworms and clams 3. The evolution
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