second Petri dish‚ which had plasmids but no antibiotic‚ also had positive results. The third Petri dish had no plasmid but did contain the antibiotic. No growth occurred in this Petri dish. The last Petri dish had no plasmid and no antibiotic. The results were positive and growth was observed. In Petri dish 1‚ we found that colonies had formed. Each colony consists of bacteria that have been transformed and are resistant to the antibiotic because they received the plasmid. This Petri dish did glow when
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in and out several times‚ before depositing 3 drops onto the agar solution in a petri dish. Next‚ another group member used an alcohol wipe to sterilize the spore spreader. It was necessary to ensure no contamination of the spores. He then proceeded to distribute the spores around the dish and solution. After sealing the petri dish back up‚ I labeled the dish with our group name and date. Finally‚ the petri dish was placed inside of the culture dome to properly germinate. The second
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Sterile filter paper disc PROCEDURE i. The bottom of a petri dish has been labeled with the name of the organism‚ the chemical agent and name and date of experimenter and experiment respectively. ii. One or two loopfuls of the organism has been taken from the cultures provided and a tube of liquid nutrient agar has been aseptically inoculated at 50oC. The mixture has been poured into a sterile petri dish. iii. When the
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second agar plate‚ open the “stick” end of the sterile cotton swabs to avoid contamination of the swab. Deep the swab in to the sterile water and collect a dust form the corner of the table by swab and rub the swab over the entire surface of the Petri dish without going back over areas you have already swabbed. 3. For the third agar plate‚ divide the plate in two different parts like washed and unwashed finger tip and swipe on each side of plate‚ see the difference between them. 4. For the forth
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Aim: To investigate the effect to the potato cells in the different solute concentration water Introduction; Water can move through the different cells due to the difference of water potentials in the cells. If there is a higher solute concentration in the cell than outside the cell‚ the water will move into the cell. However‚ if the concentration of inside the cell is lower than the outside‚ water will not move into the cell. This process is called osmosis. Research question; This investigation
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INTODUCTORY PLANT PATHOLOGY C123P1 Isolation & Study of Sclerotinia (Monilinia) Fructigena Extraction of Polygalacturonase(PG) enzyme Assay Sclerotinia (Monilinia) Fructigena • 10g of infected tissue was taken and ground in a mortar and pestle with 100Mm PH5 citrate buffer then filtered through 4 layers of muslin. • Half of this mixture was boiled at 50°C for 10 minutes and the other half was left at room temperature. • The above steps were repeated with a sample with healthy
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How to Carry Out Aseptic Techniques in a Batch Culture and in the Laboratory | | | | | | | | | |The batch vessel should be sterilised beforehand using steam. The nutrient medium that is added to the vessel |
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How would you compare the life of the poor Egyptian boy in ‘The Conjurer Made off with the Dish’ with that of a poor boy living in a metropolitan city like Karachi? “The day was passing and soon mysterious darkness would descend”. This is how Naguib Mahfouz’s story ends. A short story filled with a lifetime of experiences that perhaps every poor boy in an urban city faces. Experiences of violence‚ sex‚ power struggle‚ poverty‚ failure‚ love‚ hard choices are what make this story of a young
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petri dishes at a tilted angle‚ with the petri dish lids at an angle as well to reduce airborne contamination. After the end of each pour—no more than 2/3 of the petri dish’s capacity—the flask containing the liquid media was sterilized briefly through the Bunsen-burner by running it through the flames a couple of times for a few seconds‚ then the aluminum foil was also briefly sterilized by this same method. These lids were closed on the petri dish and left
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Introduction- The purpose of this experiment is to obtain isolation of individual species of particles from the mixed culture. This is completed through the isolation technique of streak plate. The objective of this experiment is to replicate the technique of streak plate but on a much larger scale. Because it is on a larger scale the particles are able to be visually observed as they are isolated using the streaking technique as the experiment is conducted. The benefits of the streaking technique
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