The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all very specific as each enzyme just performs one particular reaction. Catalase
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stability under several conditions. To do this‚ we tested effects on peroxidase activity‚ first‚ with different amounts of the enzyme‚ next at temperatures of 4oC‚ Room Temperature‚ 32oC‚ 48oC and boiling; then‚ at pH 3‚ pH 5‚ pH 7 and pH 9; and‚ finally‚ with the competitive inhibitor‚ hydroxylamine. We were able to measure enzymatic activity by the change in absorbance per second with a spectrophotometer. By testing different concentrations of peroxidase and its reaction rate in seconds‚ we were
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The purpose of this investigation was to determine which out of five readily available chemicals was the most effective at scarifying seeds to improve the rate of germination‚ and what concentration of the most effective chemical was the most efficacious. Ten seeds for each of the five chemicals hydrogen peroxide‚ glucose‚ hydrochloric acid‚ isopropyl alcohol and citric acid were soaked in the solution for approximately twelve hours then subsequently rinsed to remove residue of the chemicals. They
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The Effect of Temperature on Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how
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Investigation Aim: The aim of the investigation was to investigate the effect of substrate concentration Hydrogen Peroxide H O (in %) on the rate of reaction of the enzyme catalase (in 1/mean time). Prediction: As the substrate concentration (hydrogen peroxide) in % increases the rate of reaction in 1/mean rate increases until the solution becomes saturated with the substrate hydrogen peroxide. When this saturation point is reached‚ then adding extra substrate will make no difference. The
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Study of the first element – Hydrogen. Answer the following questions:- 1. Give a reason why hydrogen can be placed in group 1[IA] and group 17[vIIA] of the periodic table. 2. What similarities does it show with group 1[IA] and group 17[VIIA]. With special reference to valency electrons and ion formaton and examples. 3. How does hydrogen occur in the free and combined state? 4. Which metals react with cold‚steam and boiling water to form their respected oxides and hydroxides ? Give examples and
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LAB: Substrate Concentration Affecting the Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because
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Determining the Concentration of an Unknown Solution Graph: The effect of concentration of CuS04 * 5H20 on transmittance. I solved for my unknown by plugging in “y” as my given transmittance value‚ which was 85.0. y = -278x + 100.12 .85 = -278x + 100.12 -99.27 = -278x X = .357M (concentration) The relationship between the transmittance values and the concentration is an indirect relationship. Whenever the concentration increases‚ the transmittance decreases. It can also be reversed
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INTRODUCTION Osmosis is the movement of water molecules from high concentration to low concentration through semipermeable membranes‚ caused by the difference in concentrations on the two sides of a membrane (Rbowen‚ L.). It occurs in both animals and plants cells. In human bodies‚ the process of osmosis is primarily found in the kidneys‚ in the glomerulus. In plants‚ osmosis is carried out everywhere within the cells of the plant (World Book‚ 1997). This can be shown by an experiment with potato
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Lab Report 3: Combustion of Magnesium and Specific Heat of a Metal C4C Jeffrey Silvin Fall 2017 Major Kittle Chem 100 T1 Introduction The purpose of this lab was to determine an experimental value for the heat of formation of MgO with Hess’s Law and then use the result to find percent error. To do so‚ approximately 50 mL of 1.0 M HCl was added to a calorimeter. Initial temperature was measured and then 0.25 g of Mg was added. After the reaction is completed the maximum temperature was recorded.
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