Effects of Temperature‚ pH‚ Boiling‚ and Hydroxylamine on the Enzyme Peroxidase Extracted From Brassica rapa Abstract In this experiment the enzyme peroxidase was extracted from from a turnip‚ Brassica rapa‚ and tested under different conditions. The effects of temperature‚ boiling‚ pH‚ and a competitive inhibitor were tested. The enzyme was tested at temperatures of 4°C‚ 24°C‚ 32°C‚ and 48°C. As the temperature increased‚ so did the activity of the enzyme
Premium Enzyme Oxygen Catalase
Title: Enzyme Activity Lab Purpose: To measure the rate of enzyme activity from a tissue abstract and experiment with different factors‚ such as the enzyme solution and the substrate with different hydrogen peroxide percentages and temperature‚ that affect enzyme activity. Hypothesis: 1) If the disk is placed into each beaker with 100 units/ml of enzyme solution‚ then the time for the disk to float will be 30 seconds. 2) If the temperature of the solution is at 5 degrees Celsius‚
Premium Enzyme Chemical reaction Oxygen
FOOD NUTRITION BASICS LABORATORY REPORT LAB 3 PROTEINS DUE DATE Next FNB Practical NAME (CAPITALS) ______________________________GROUP LETTER___________ This work is the product of my own efforts and has not been copied from any other sources except where full acknowledgement has been given. Signed________________________________________ Student No._____________ Introduction The principle involved in this experiment is denaturation. It involves loss of ordered structure and can be caused by changes
Premium Protein Egg white Milk
Hydrogen has a high specific energy‚ high flame speed‚ wide range of flammability‚ and clean burning characteristics which suggest a possibility of high performance in internal combustion engines (ICE). These attributes have been realized for more than half a century since the onset of hydrogen engine development. In the early 1990s‚ FSEC conducted research on using hydrogen in an ICE. This work resulted in the development of a mixed fuel called HYTEST. Today‚ automobile manufacturers and DOE continue
Premium Internal combustion engine
In the humus lab were were trying to find out if hydrogen peroxide would cause a reaction and show us the amount of humus in the soil by putting gas into a graduated cylinder with water to make the water level lower therefore showing us how much humus the material being tested contained. It showed that the sand was lacking humus but the mushroom compost was the most rich in humus. My hypothesis was correct because I predicted that the mushroom compost would produce the greatest amount of humus out
Premium Water Oxygen Chemistry
Ashley Esparza Stallings Period 2 January 26‚ 2013 Enzyme Catalysis Lab Report Background: Enzymes are catalyst‚ which affect the rate of a chemical reaction. One consequence includes the cell to carry out complex chemical activities at relatively low temperatures. In these reactions the substrate binds reversibly to the active site. The cause of this is a decrease in the energy needed to activate the reaction of the substrate molecule to from products. Every enzyme is particular for a
Premium Enzyme Catalysis Chemical reaction
LAB 10: NAME: DaeNia La Rodé DATE: 25TH January‚ 2011. FORM CLASS: L6 3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of substrate concentration on the enzyme amylase INTRODUCTION: Enzymes are perhaps one of the most important proteins of the human body. Enzymes such as amylase‚ an enzyme that breaks down carbohydrates‚ work by means of surface catalysis. In other words‚ the surface of the enzyme enables other molecules to react in a manner they would not be able to without
Free Enzyme Starch
LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
Premium Plasmid Transformation Escherichia coli
Graduated Cylinder (10mL) Beakers (250mL) Buret stand Erlenmeyer flask (125mL) Safety goggles Buret clamp 0.10M HCl (aq) (25.0mL) NaOH (aq) Vocab: Standard Solution: a solution containing a precisely known concentration Titration: a measured amount of a solution of unknown concentration is added to a known volume of a second solution until the reaction between them is just complete Endpoint: The part in
Premium Titration Sodium hydroxide Acid
we performed two different experiments that investigated the effect of varying substrate concentration‚ and the effect of temperature on the rate of Enzyme-Catalase reaction. In experiment one (i.e. the effect of varying substrate concentration on the rate of enzyme-catalase reaction) we tested the hypothesis HA as substrate concentration increases‚ so will reaction rate until all active sites are bound. In experiment two (i.e. The effect of temperature on the rate of enzyme-catalase reaction) we tested
Premium Enzyme Chemical reaction Catalysis