The effect on rate of an enzyme catalysed reaction by different objectives which include effect of substrate‚ temperature‚ ph and effect of a competitive inhibitor phosphate ions. This is determined by the reaction of hydrolysis by p-nitrophenylphosphate (PNP) as a substrate by the enzyme phosphatase. Abstract The hydrolysis of p-nitrophenyl phosphate has been studied in human red blood cells. To see if hydrolysis was related to the functioning of the sodium pump. Acid phosphatase catalysis’s
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Abstract The catalysis of enzymes within our bodies is essential for human survival and when this ability is impaired by the presence of Hydrogen ions‚ our cells cannot function properly. This experiment was conducted to determine if the reaction rate changes in response to a variation of acidic‚ neutral‚ and basic solutions. The experimental results indicated that the basic/high pH solution has a faster rate of reaction in the solution. Introduction Enzymes are proteins that catalyze and
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Cellular respiration is defined as an enzyme mediated process in which organic compounds such as glucose is broken down into simpler products with the release of energy (Duka‚ Diaz and Villa‚ 2009). It is a series of metabolic processes and oxidation-reduction reactions. Oxidation of substrates‚ such as glucose‚ is a fundamental part of cellular respiration (Mader‚ 2009). As a catabolic process‚ it may or may not require the presence of oxygen. The process that requires oxygen is called aerobic
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25 April 2000 NAME 1. [10 points] What are the concentrations of HSO− ‚ SO2− ‚ and H+ in a 0.20 M KHSO4 solution? (Hint: 4 4 H2 SO4 is a strong acid; Ka = 1.3 × 10−2 for HSO− .) 4 2. [15 points] Calculate the pH of 1.00 L of the buffer 1.00 M CH3 COONa/1.00 M CH3 COOH (pKa = 4.74) before and after the addition of (a) 0.080 moles NaOH and (b) 0.12 moles HCl. (Assume there is no change in volume). 3. [10 points] The following reaction is found to be first order in A: A −→ B + C If half
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Enzymes are proteins or nucleic acids that catalyze reactions. They are able to speed up reactions by reducing the activation energy of a reaction. Each kind of enzyme has a specific shape that matches its substrate so it can bind to its active site. Enzymes convert their substrates into a product. Enzyme activity are affected by factors such as temperature‚ pH‚ and time. If an enzyme is exposed to extreme heat‚ it will become denatured‚ that is‚ to become deformed and lose its original shape which
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Introduction “The Enzyme Reaction” An enzyme is a protein that acts as a catalyst‚ which brings out a biochemical reaction. A Catalase enzyme‚ the enzyme tested in this experiment‚ is found in almost all living organisms that are exposed daily to oxygen (such as fruits‚ vegetables and animals). Background Information The Catalase enzyme in this experiment is known for being less affective the warmer the temperature is. According to “Science fair projects” an enzyme becomes unstable
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Kinetics 6.1 Rates of reaction 6.2 Collision theory 6 16.1 Rate Expression (AHL) 16.2 Reaction mechanism (AHL) 16.3 Activation energy (AHL) 6.1 Rates of reaction 6.1.1 Define the term rate of reaction. 6.1.2 Describe suitable experimental procedures for measuring rates of reactions. 6.1.3 Analyse data from rate experiments. © IBO 2007 Figure 601 An explosion is a quick reaction D ifferent chemical reactions occur at different rates (i.e. speeds). Some
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RATE OF REACTIONS. The reaction rate (rate of reaction) or speed of reaction for a reactant or product in a particular reaction is intuitively defined as how fast or slow a reaction takes place. For example‚ the oxidative rusting of iron under the atmosphere is a slow reaction that can take many years‚ but the combustion of cellulose in a fire is a reaction that takes place in fractions of a second (right). Chemical kinetics is the part of physical chemistry that studies reaction rates. The concepts
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Aim: To determine the effect of altering concentration (mol dm-3) of hydrochloric acid (HCl) on the rate of reaction (mol dm-3 s-1) for the following chemical reaction: CaCO3(s) + HCl(aq) CaCl2(s) + H2O(l) + CO2(g) Research question: How does altering concentration (mol dm-3) of hydrochloric acid (HCl) affect the rate of reaction (g/s) for the following reaction: CaCO3(s) + HCl(aq) CaCl2(s) + H2O(l) + CO2(g) Background information: The reaction between calcium carbonate and hydrochloric acid
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Enzyme Controlled Reactions 1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases? A) The relationship between the substrate concentration and the initial reaction of an enzyme-catalyzed reaction is very productive‚ but is dramatically affected by the pH level of the given solution. The most productive pH level is
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