SYNTHETIC EXPLOITATION OF ENZYMES: BIOCATALYSIS IN ORGANIC SOLVENTS: FUNDAMENTALS ENZYMES IN ORGANIC SYNTHESIS 1. Enzymes catalyze a broad spectrum of reactions with high turnover numbers. Rate enhancements approach 1012 fold. 2. Enzymes may accept a wide range of substrates. 3. Enzymes are highly regio and stereoselective. 4. Enzyme reactions take place under mild conditions; this minimizes problems of isomerization and racemization. 5. Enzymatic processes are less hazardous and polluting
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Investigation the effect of the organic solvents on the cell membrane of the beetroot Objective: To investigate the effect of different organic solvents‚ such as alcohol and paraffin oil‚ on the cell membrane of the beetroot by using the red pigments in the beetroot as indicator. Hypothesis: Organic solvents dissolve the organic matter in the cell membrane (such as phospholipids). This destroys the cell membrane‚ and the permeability of the cell membrane is disrupted casing red pigments to
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TEACHER NOTES & GUIDELINES Title of Lab: Effects of Temperature and Solvents on the Cell Membrane Developers of Lab: Adapted by James Kirby JD726‚ Jennifer Mortellaro JD449‚ and James Prockup JD575 from a publication by the Department of Biological Sciences at Western Michigan University‚ Kalamazoo‚ MI Overview of Lab: Description: The purpose of this lab is to illustrate the effects that temperature and solvents have on the cell membrane. Red beet tissue contains large
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Extraction is a method used for separating and isolating materials. A compound is pulled from solvent A to solvent B and in this case‚ both solvents are miscible. These two concepts are not capable of mixing together; when they are mixed together‚ they form a bilayer. This can be seen when mixing oil and water together. When thinking of extractions‚ one can picture how coffee gets extracted. The flavors‚ molecules‚ caffeine‚ etc. are extracted from the coffee beans. In this experiment‚ a solution
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Stress and Cellular Membranes Bio Lab SBI 4U1 Due: Sept 24th‚ 2010 For: Ms. Barton By: Ameema Saeed Introduction: (See attached lab) Purpose: (See attached lab) Hypotheses: Tap water will cause no damage to the membranes. The extreme temperatures of the water cooled to 0°C‚ and heated to 94-100°C will greatly damage the membranes. Extreme heat will be more damaging than extreme cold. The more concentrated the solvent‚ the more damage it will cause to the membranes‚ therefore the
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The Effect of Solvent Exposure and Temperature on Beet Cell Membrane Integrity Introduction: • Cell membranes are vital to proper cellular function • Cell membranes consist of a lipid bilayer made up of phospholipids • Phospholipids are amphipathic molecules meaning they have a dual nature in that they show both hydrophilic (water-loving) and hydrophobic (water-fearing) properties • The amphipathic nature of phospholipids causes them to spontaneously form bilayers in water
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Practical 2.1- The Effect of Temperature on Membranes Objective To investigate the effect of temperature on membrane structure Introduction Beetroot Pigments Beetroots contain Betalains which are the red pigments present in the cell vacuole. Betalains are soluble in water and they contain nitrogen. Betalains extracted from beetroot is commonly used as food dye because it is not known to cause any allergic reactions. Beetroot Picture taken from http://tipdeck/how-to-cook-beet-root Structure
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chemicals that give it strength‚ flexibility‚ and resistance to chipping and peeling. WHAT IS IT MADE OF? 1. First‚ solvents are used to mix together all the ingredients in a nail polish to yield a uniform liquid product. a. The type and amount of solvent determines how thick a polish is and how long it takes to dry b. Most Common solvent- ethyl acetate. c. Ill talk more about what the solvent does in a few slides. d. See pic 2. Another ingredient are resins- a polymer material that holds the polish
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on determining the stress that various alcohols have on biological membranes. Using five solutions of differing alcohol concentration for each of the three alcohols; methanol‚ ethanol‚ and 1-propanol and a small slice of beet‚ the stirred solution was placed into a plastic cuvette and then into a spectrophotometer and the absorbance of alcohol solutions were determined in order to conclude which alcohol and concentration of alcohol had the greatest effect on biological membranes. The results showed
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Introduction To determine the effects of stressful experimental treatments on living membranes we are going to examine how fresh beets roots react when they are exposed to different temperatures. Membranes are an important feature of plant cells and they act as a barrier that separates the interior of the cell from the external environment (Campbell 133). They organize specific chemicals and reactions into specific compartments within the cell. Generally‚ cell membranes consist of phospholipids bilayer
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