Investigate Concentration of Substrate on Catalase enzyme 1. Aim/Prediction. Aim of this experiment is to investigate concentration of substrate on catalase enzyme‚ as well to see how they work in different concentrations of hydrogen peroxide (H2O2). Prediction would be‚ that the filter paper discs that were used‚ will rise up to the surface in a time in the tube of different concentration of hydrogen peroxide. And in different concentrations the time will be different. As higher the concentration
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Biology Enzyme Catalase Investigation Aim: The aim of this investigation is to study and observe whether or not the concentration of hydrogen peroxide (varying from 10 – 30 millilitres) affects the rate of reaction. Hypothesis: With the increase of the concentration of Hydrogen Peroxide 3% substrate‚ I prediction that the rate of pressure increase will begin to amplify. The pressure is bound to increase because the catalase quickly reacts with the hydrogen peroxide; this is why the more substrate
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Dana Calderone Responses of Enzyme Activity from pH and Concentration Abstract Enzymes are the key to many of the chemical reactions that our bodies depend on to live. Without enzymes‚ we would not exist. These biological catalysts speed up the reactions as well as reduce the amount of activation energy needed to complete the process. Knowing how important enzymes are to us‚ it is important to realize what they require to function. They need select conditions and rates to work right. These conditions
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reaction involves enzymes. So to understand how bleach works‚ we first understand how enzymes work. Enzymes Enzymes are the most important thing in our lives‚ it makes the world go ‘round! Enzymes are proteins that acts as catalysts and help combine or break down hydrogen peroxide. Imagine a pen with ink inside it‚ when you write‚ ink comes out‚ right? You can draw‚ you can write‚ then when you finish and get your pen off of the paper‚ the ink stops coming out. Enzymes are like that‚ they
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of an enzyme-controlled reaction. How do these factors affect the chemical structure and properties of the enzyme. Many things can affect the rate of enzyme activity. The temperature of the enzyme‚ the pH of the solution‚ the concentration of the enzyme‚ substrate and the product. Also‚ another affector is the number of competitive and non-competitive inhibitors. As I cannot explain them all‚ I have chosen to explain the effect of temperature and also the effect of inhibitors on enzyme activity
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relationship between catalase concentration and the rate of reaction. This means when catalase concentration increases‚ the rate of reaction also increases. In other words‚ at 20% catalase concentration‚ the rate of reaction was only 4.220 mm/s while at 100% catalase concentration‚ the rate of reaction was 7.704 mm/s. This proves the positive correlation between catalase concentration and the rate of reaction. This occurs because as the enzyme concentration increases‚ there are more enzymes available to
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Effects of Temperature and pH on Enzyme Function Chelsie Mesa Section 0479 Robin Cotter Introduction The purpose of this experiment is to identify three unknown enzymes. This is done by using different temperature and pH to affect the function of the enzyme‚ which ultimately‚ will affect how much maltose is produced. Enzymes are proteins that help catalyze chemical reactions. In enzymatic reactions‚ the molecules at the beginning of the process are called substrates‚ and the enzyme
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pH and Microbial Growth Introduction: Each organism has an optimum environment for growth‚ which includes pH level. Organisms that grow at near neutral pH levels are called neutrophiles. Those that grow best in acidic pH values are called acidophiles. Others‚ yet‚ grow best in alkaline pH values and are called alkaliphiles. In this lab‚ each group was assigned an organism‚ which was then used to inoculate 6 different pH levels. Materials and Methods: Followed lab procedure 29‚ “pH and
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this unknown sample. First‚ the plate was analyzed for its hemolysis characteristics. Afterwards‚ a Gram stain was performed from a sample of the agar plate and the slide was viewed under the microscope. Once‚ the microscopic visual was captured‚ a catalase test followed. Next‚ for further data‚ MSA results were recorded‚ along with antibiotic observations of demo plates. Lastly‚ with the information gathered‚ it was identified that the unknown sample was Streptococcus salivarius. Introduction:
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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