Lab #6: pH Effects and Enzyme Concentration on Catecholase Activity Abstract: This experiment examined the effect on how the changes in pH balance of potato juice affected the enzyme activity‚ structural stability and solubility. Introduction: Enzymes are macromolecules that allow chemical reactions to occur. They function as biological catalysts. A catalyst is a substance that is involved in‚ but not changed by or consumed in‚ a chemical reaction. The amount of energy required for a reaction
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- - - - - - Effect of Substrate Concentration on Catalase Aim: An experiment to find out how the concentration of hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information: Enzymes such as catalase are globular protein molecules with catalytic properties. A catalyst is a substrate which can alter the rate of reaction without itself undergoing any permanent change. As they are not changed my reactions which they catalyse‚ enzymes can be used
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Understanding how catalase activity is affected by pH is the purpose of this experiment. Introduction Enzymes play an important role in daily life because of the chemical reactions. Almost chemical reactions require the presence of enzymes to promote the metabolic process. They are known as the incredibly efficient and highly specific biological catalysts. Most enzymes are protein with the ability to enhance the rate of reaction between molecules. To catalyze a reaction‚ the enzymes have to create
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Abstract In this laboratory exercise we studied enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment
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How does pH affect the activity of the Amylase? Abstract: In my experiment I aimed to observe how ranging pH levels will affect the rate in which amylase will break down the starch molecules. I will be measuring the time it takes for the dark liquid to disappear and leave a yellow brown liquid to be shown‚ which would show that there is no starch present in the solution because it would have broken into maltose by adding amylase. Results did not fully demonstrate what we expected in our hypothesis
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lab is to determine the pH level of common household products. Introduction Liquids are either acidic‚ basic‚ or neutral. We are able to figure out which one it is based on properties‚ along with the pH. Acids taste sour‚ and bases taste bitter. Using the pH scale‚ you are able to know how strong of an acid or base a liquid is. High concentration of [H+] ions means that the liquid has a low pH. Therefore a liquid with a low concentration of [H+] would have a high pH. A pH of less than 7 represents
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Assessment of Catalase Function Lab Introduction The purpose of this lab report was to test and measure the rate of substrate destruction by an enzyme‚ we tested the destruction of hydrogen peroxide by the enzyme catalase. Hydrogen peroxide is a poisonous by product of metabolism that can damage cells if it is not removed. Catalase is an enzyme that speeds up the breakdown of hydrogen peroxide into water and oxygen gas. H2O2 + catalase → H2O + O2 A catalyst is a substance that lowers the
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peroxide and catalase. Tests were performed by putting chicken liver‚ ethanol solution (diluted ethanol solution for other trials) and hydrogen peroxide in a test tube with a side arm‚ and having a rubber tube lead the oxygen gas into a gas collection tube. Results from the tests showed a negative correlation‚ this means that the more diluted the solution of 95% ethanol was‚ the less oxygen gas collected. Controlling a number of factors which include human error‚ temperature change‚ pH levels‚ substrate
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investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different enzyme can be found in any cell. Enzymes can be describe
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Effect of an Orange on Catalase Activity in Comparison with a Potato Introduction Catalysts are substances that increase chemical reactions while using less energy. They also remain unchanged after use‚ which enables it to be used repeatedly. Cells have special catalysts called enzymes‚ which are specialized proteins that help accelerate chemical cell reactions. (Evert‚ RF & Eichhorn‚ SE 2013). Enzymes also control plant metabolic processes such as respiration (Evert RF‚ Eichhorn SE & Perry JB
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