Effects of Temperature and pH on Catalase Activity INTRODUCTION Enzymes are organic catalysts that spur metabolic reactions. The presence of an enzyme within a cell is essential in order for any sort of reaction to take place. All enzymes are complex proteins that act in an organism’s closely controlled internal environment. In such a homeostatic environment‚ the temperature and the pH (concentration of hydrogen ions)‚ remain within a fairly narrow range. Extreme variations in pH and temperature
Premium
Effects of Enzyme Catalysis of H2O2 by Catalase Report by: Timmy Lin (#269164729) October 17‚ 2011 Mr. Rienzi AP Biology Problem: Measuring the effects of Catalase enzymes on hydrogen peroxide decomposition. Measuring the rate of the reaction when hydrogen peroxide and Catalase are mixed at the same ratio for different time (10‚ 20 30 60 120 180 360 seconds). Background: Enzymes are biological catalysts that carry out cellular metabolic processes with the ability to enhance the rate
Premium Oxygen Enzyme Hydrogen peroxide
Finding the Optimal pH of the Enzyme Peroxidase with the Aid of a Spectrophotometer ABSTRACT The peroxidase enzyme was partially purified‚ and the enzyme activity was calculated with the use of a spectrophotometer‚ demonstrating the effect of pH on peroxidase activity. The objective of this exercise was to determine the ideal pH for peroxidase activity. The data concluded that the optimum pH for peroxidase activity is 5‚ and the “standardized” enzyme volume is 2.8mL.
Free PH
Part B: Practical Report The Effect of Temperature on Enzyme Activity Aim: To investigate how temperature effects the enzyme catalase. Hypothesis: If the temperature of water is increased then the enzyme will react quicker to form oxygen and water‚ when compared to cold water. Purpose: To design and conduct a plan of a practical about the effects of temperature on enzymatic activity with a partner. Introduction: An enzyme is a protein‚ which speeds up a specific chemical reaction without altering
Premium Catalysis Chemical reaction Enzyme
Temperature Affecting Enzyme Activity Introduction The basic properties of life revolve around chemical reactions. Without the presence of enzymes some of life’s processes would not come so easily. Enzymes are basically proteins‚ which have specific shapes for different substrates. Enzymes change the rate in chemical reactions. It does this without having to change its own shape‚ which makes enzymes different from other proteins. A common enzyme that we have is catalase‚ which breaks down hydrogen
Premium Temperature Chemical reaction Enzyme
The Effect of pH on a Food Preservative September 18‚ 2012 Objective: To study the affect of pH on a food preservative. Chemical Equation: C6H5COONa + HCL ------ C6H5COOH + NaCl Procedure: This experiment was started with a clear solution of sodium benzoate and HCl was added to it‚ ultimately producing benzoic acid. First‚ .3395 g of sodium benzoate was weighed‚ then it was dissolved in water‚ causing it to disassociate into ions. Next‚ 3M of HCl were added drop wise to the solution
Premium Solubility Acid Sodium benzoate
From performing the pH experiment it has proven that pH buffer 7 is the optimum pH for catalase in potatoes. This is because the height of the bubbles was the highest of all three tests (pH 3‚ pH 7‚ pH 9) reaching an average height of 0.433 cm. See graph 3. While pH buffer 3’s average height was 0.233 cm only reaching a maximum height of 0.3 cm in Trial 3 and pH buffer 9’s average height being 0.333 cm only reaching a maximum height of 0.4 cm again in Trial 3. See table 1. Therefore this supports
Premium Enzyme PH Temperature
From the graphs‚ it is evident that an increase in both catalase concentration and substrate concentration resulted in a higher rate of reaction or‚ as observed in the kPa graphs‚ a higher volume of O2(g) formed at the end of the 5 minute trial. Interestingly‚ it should also be noted‚ as it was mentioned in the Figure 2‚ that the trend for the 6mL of 3% H2O2(aq) was more of a linear trend than an exponential decay‚ steadily rising until the end of the 5 minute trial. From this‚ it can be inferred
Premium Enzyme Chemical kinetics
TITLE: Enzymes AIM: To investigate the effect of temperature on the enzyme lipase INTRODUCTION: The phenomenon of catalysis makes possible biochemical reactions necessary for all life processes. Catalysis is defined as the acceleration of a chemical reaction by some substance which itself undergoes no permanent chemical change. The catalysts of biochemical reactions are enzymes and are responsible for bringing about almost all of the chemical reactions in living organisms. Without enzymes‚ these
Premium Enzyme PH PH indicator
The effects of pH on mung beans Gloria Cheng Winsy Cheung Lily Wong Christine Yen January 15‚ 1998 Abstract This experiment explores how different pH environments affect the growth of mung beans. The mung beans were grown in water with various pH levels‚ consisted of pH levels 5‚ 6‚ 7‚ 8‚ and 9. A replicated design was used consisting of 3 runs for each pH level. The following results are listed in order of pH levels allowing most growth to least growth of the mung beans: 7‚ 8‚ 6‚ 5‚ and 9. These
Premium PH Acid