Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab‚ click on the Experiment button to begin the first assignment. This assignment is designed to help you become
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optimum pH of 10.0 with the liberation of p-nitrophenol. The substrate is colourless‚ but the product p-nitrophenol is yellow in alkaline solution‚ absorbing maximally at 405 nrn. Thus a convenient assay for this enzyme involves monitoring the change in absorbance of the reaction medium at 405 nm. Exergonic (i.e energy producing reactions) exhibit a negative free energy change. Sometimes these reactions occur spontaneously‚ but generally some energy must be supplied to initiate the reaction; in
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Top of Form 1. What is the pH of a buffer that results when 33 g NaHCO3 is mixed with 100.0 mL of 2.50 M NaOH and diluted with water to 250 mL? (Ka for HCO3- = 4.8 10-11) (Points : 10) 8.20 10.07 10.12 10.32 10.56 2. What is the pH of an aqueous solution of 0.30 M HF and 0.15 M F-? (Ka of HF = 7.2 10-4) (Points : 10) 1.83 2.84 3.14 3.44 10.86 3. Which of the following mathematical expressions
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Reaction Order and Rate Law Name Data‚ Calculations‚ and Questions A. Calculate the initial and final concentrations as needed to complete Tables 1 and 2. Data Table 1: Varying the Concentration of 1.0 M HCl | | | | |Concentrations | | |# Drops |# Drops |# Drops |Initial
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Determination of Rate and Order of a Reaction Results This experiment used a spectrometer to find the wavelength with maximum absorbance in a green food coloring solution. For this particular solution the wavelength was 629.7 nm. The system was then calibrated to that and was set to measure the food coloring and bleach solution. The measured visible light absorbance of the mixed solution was collected over a time of 200 seconds and eight points were then selected and placed into the Absorbance
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investigate the effect of different temperatures on the activity of rennin in milk Introduction Enzymes are globular protein‚ responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon‚ hydrogen‚ oxygen and nitrogen (Gunsch‚ 2012). The role of enzyme is to act as catalysts‚ substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done by lowering
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3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of substrate concentration on the enzyme amylase INTRODUCTION: Enzymes are perhaps one of the most important proteins of the human body. Enzymes such as amylase‚ an enzyme that breaks down carbohydrates‚ work by means of surface catalysis. In other words‚ the surface of the enzyme enables other molecules to react in a manner they would not be able to without the surface of the enzyme present. Enzymes achieve this by lowering the
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Lab 3: Reaction Order and Rate Laws Name: Student Lab Partners: None Date of Experiment: 16 March 2014 Location: My House Course Number: CHE112 Abstract: When combining compounds‚ there are always two roles considered in the reaction. These are reactants and products. Reactants are the initial compounds before mixing. The products are synonymous with the term byproduct‚ or what remains when the reaction occurs. For example‚ Sodium (Na+) and Chlorine (Cl-)
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An international journal published by the Nigerian Society for Experimental Biology Printed in Nigeria Cofactor interactions in the activation of tissue non-specific alkaline phosphatase: Synergistic effects of Zn2+ and Mg2+ ions Femi J. OLORUNNIJI*‚ Adedoyin IGUNNU‚ Joseph O. ADEBAYO‚ Rotimi O. ARISE and Sylvia O. MALOMO Department of Biochemistry‚ University of Ilorin‚ P.M.B. 1515 Ilorin‚ Nigeria Received 19 March 2007 MS/No BKM/2007/028‚ © 2007 Nigerian Society for Experimental
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results. This is due to the enzymes being most active at 60ᵒC‚ it was that results were going to be most active at the 41ᵒC which is close to human body temperature. When we compare the enzyme in the low temperatures to the enzymes in the higher temperature we observed that the enzymes were functioning at a significantly reduced rate at the 24ᵒC temperature and was completely inactive at the 0ᵒC temperature. Whereas when we compare to the higher temperatures (60ᵒC) the enzymes should have functioned
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