2. [15 points] Calculate the pH of 1.00 L of the buffer 1.00 M CH3 COONa/1.00 M CH3 COOH (pKa = 4.74) before and after the addition of (a) 0.080 moles NaOH and (b) 0.12 moles HCl. (Assume there is no change in volume). 3. [10 points] The following reaction is found to be first order in A: A −→ B + C If half of the starting quantity of A is used up after 56 seconds‚ calculate the fraction that will be used up after 6.0 minutes. 4. [15 points] The rate law for the decomposition of ozone
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_____________________________________________________________________ Investigating Factors that Affect the Rate of Enzyme Activity See Textbook Pg 61. Enzymes are very large complex organic molecules that are synthesized by the cell to perform specific functions. These biological catalysts are important because they speed up the rate of the reaction that would otherwise be too slow to support life. Catalase is an enzyme present in the cells of plants‚ animals and aerobic (O2 requiring) bacteria. It promotes the
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Practical: The effect of pH on enzyme activity Rationale Amylase is an enzyme involved in the digestion system which catalyses the breakdown of starch into sugars. It is not only present in human saliva but also in the pancreas‚ where it hydrolyses dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. pH has an effect on the activity of all enzymes‚ including amylase. As the pH level increases‚ the enzyme activity increases
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An Investigation on the rate of reaction of the enzyme Catalase on the substrate Hydrogen peroxide. Plan Aim: To investigate the rate of the effect of Catalase on hydrogen peroxide. Introduction This investigation will be carried out to investigate the rate of reaction of the enzyme catalase on the substrate hydrogen peroxide. Enzymes are biological catalysts‚ which speed up the rate of reaction without being used up during the reaction‚ which take place in living organisms. They do this by
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Enzymes are biological catalysts that speed up chemical reactions by lowering the amount of activation energy needed to start the reaction and let the reaction occur at temperatures found in living cells. The way that enzymes do this is explained with the lock and key hypothesis. This hypothesis says enzymes have a specific shape called the active site which is different between different enzymes. Molecules called the substrate that participates in the reaction also have a specific shape that can
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RATE OF REACTIONS. The reaction rate (rate of reaction) or speed of reaction for a reactant or product in a particular reaction is intuitively defined as how fast or slow a reaction takes place. For example‚ the oxidative rusting of iron under the atmosphere is a slow reaction that can take many years‚ but the combustion of cellulose in a fire is a reaction that takes place in fractions of a second (right). Chemical kinetics is the part of physical chemistry that studies reaction rates. The concepts
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The Effect of pH on Enzymatic Reaction Rate Abstract Enzymes are affected by changes in pH. The most favorable pH value— the point where the enzyme is most active—is known as the optimum pH. This experiment was conducted to determine the effect of pH reaction rate on an enzyme‚ catalase‚ from yeast. The experimental results indicate that the catalase worked best at a neutral pH level of seven (7). Introduction An enzyme is a protein molecule that serves as a catalyst. “The basic function of
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Introduction “The Enzyme Reaction” An enzyme is a protein that acts as a catalyst‚ which brings out a biochemical reaction. A Catalase enzyme‚ the enzyme tested in this experiment‚ is found in almost all living organisms that are exposed daily to oxygen (such as fruits‚ vegetables and animals). Background Information The Catalase enzyme in this experiment is known for being less affective the warmer the temperature is. According to “Science fair projects” an enzyme becomes unstable
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ID:0720052 Effect of an Increasing Substrate Concentration on Enzyme Activity Rate Abstract The reaction rate of an enzyme can be affected by many factors‚ and the purpose of this experiment was to find out how an increasing substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of potato juice (enzyme oxidase) and different amount of catechol (substrate) while holding pH and temperature
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of ATP‚ Adenosine Triose Phosphate. This occurs by converting glucose into pyruvate via glycolysis. Pyruvate is then transported into the mitochondrial matrix via active transport. Numerous reactions‚ catalyzed by a multi enzyme complex then occur‚ where the pyruvate is decarboxylated as shown in the reaction below. Pyruvate + NAD* + CoA ? acetyl CoA + reduced NAD + Carbon Dioxide This experiment shall be carried out via the manometric technique‚ where there are two different environments with
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