3.8.3. Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature
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EFFECT OF TEMPERATURE‚PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature‚Ph and substrate concentration on the rate of enzyme activity. Hypothesis
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Armadillium vulgare taxis response to different pH levels. Abstract The terrestrial isopod‚ Armadillium vulgare is commonly referred to as a slater or pill bug. Since transitioning from the sea to land and originally colonizing in Mediterranean regions‚ it has adapted throughout evolution to inhabit local microhabitats. The pill bug is bound by several parameters and also has specific requirements that need to be met for optimal biological functioning. As such‚ behavioural and physiological
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9.5 Tests for halogens and halide Ions Test for halogen Test method Test observations Test chemistry and comments Chlorine gas Cl2 A pungent green gas. (i) Apply damp blue litmus. (Can use red litmus and just see bleaching effect.) (ii) A drop silver nitrate on the end of a glass rod into the gas. (i) litmus turns red and then is bleached white. (ii) White precipitate. (i) Non-metal‚ is acid in aqueous solution and a powerful oxidising agent (ii) It forms a small amount of chloride ion in water
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Lab #6: pH Effects and Enzyme Concentration on Catecholase Activity Abstract: This experiment examined the effect on how the changes in pH balance of potato juice affected the enzyme activity‚ structural stability and solubility. Introduction: Enzymes are macromolecules that allow chemical reactions to occur. They function as biological catalysts. A catalyst is a substance that is involved in‚ but not changed by or consumed in‚ a chemical reaction. The amount of energy required for a reaction
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Research Question: Effects of pH on amylase activity Introduction: Amylase is an enzyme that is in human’s saliva as well as the pancreas. Enzymes are biological catalysts that speed up a chemical reaction. They break down complex molecules into simple ones. In this case‚ amylase converts starches (complex molecule) into simple sugars. That is why foods like potatoes for example‚ may taste sweet to us‚ because they contain starch. The optimum pH for pancreatic amylase is the pH of 7. In the
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The purpose of this lab is to determine the rate of reaction under different circumstances. Different variables will be manipulated to discover their effects on a particular reaction. Changes in temperature‚ pH‚ and enzyme concentration are examples of factors that have the potential to affect the initial rate of an enzyme catalyzed reaction in a controlled experiment‚ whether it be speeding the reaction up or slowing it down. Part I of the experiment establishes a baseline that can be used to compare
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determine the effects of different environments affecting the rate of reaction‚ PNPP (p-nitrophenyl phosphate) + H20 ? PNP (p-nitrophenol) + H3P04. This reaction is catalyzed by the enzyme phosphatase. Different environments produced different reaction rates as environmental factors affect the efficiency of phosphatase. This is because environmental factors can change the tertiary structure of phosphatase‚ which alters its active site‚ and thus changes its efficiency to catalyze the reaction. We measured
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Once Upon a Time by Gabriel OkaraOnce upon a time‚ son‚they used to laugh with their heartsand laugh with their eyes:but now they only laugh with their teeth‚while their ice-block-cold eyessearch behind my shadow.There was a time indeedthey used to shake hands with their hearts:but that’s gone‚ son.Now they shake hands without heartswhile their left hands searchmy empty pockets.‘Feel at home!’ ‘Come again’:they say‚ and when I comeagain and feelat home‚ once‚ twice‚there will be no thrice-for then
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Enzymes are globular proteins that are synthesized by the ribosomes in a cell. They act as catalysts during biological reactions; therefore‚ enzymes are able to speed up these reactions without undergoing a permanent change themselves. These proteins are able to do this by lowering the activation energy of a reaction. To add on‚ enzymes require specific conditions under which they can work best. Reactions occur at faster rates when the temperature is higher. However‚ the rate of an enzyme-catalyzed
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