Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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Abstract The following experiment details the effect of different concentrations of catalase on the production of oxygen and water through the breakdown of Hydrogen Peroxide. In this experiment paper disc where coated in varying concentrations of catalase‚ 0‚ 25‚ 50 75 and 100%. The time taken for the disc to float between two markers on the side of a glass was then recorded. This experiment demonstrates that the higher the concentration of enzyme used the greater the production of oxygen on the
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Blank 1 Blankity Blank Blankity Blank Mr. Blank 4/9/02 Effects of Salts on the Freezing Point of Water In this experiment‚ you will study the effect that several solutes have on the Freezing Point of water. When a solute is dissolved in a liquid‚ the temperature at which that liquid freezes decreases‚ because the molecules of the solute become attached to the water molecules‚ making it more difficult for the water to form its crystaline shape and form into ice. This process is called Freezing
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Estimating Osmolarity of Plant Cells Criteria Assessed: DCP Introduction In this experiment you will be estimating the osmolarity of potato cells by finding their change in weight in solutions of known molarity. The object is to find the molarity where the mass does not change. No net change in weight indicates that there has been no net gain or loss of water. This is a means of indirectly finding the osmolarity of the cells themselves. Apparatus 1 large potato Petri dish 7 x 250 ml beakers
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RESULTS AND DISCUSSION REPORT—EXPERIMENT 3 (CHEMICAL KINETICS) CALCULATIONS Effect of Concentration on Reaction Rate [S2O32-]initand [H+]init for each run‚ knowing the original concentrations and volumes of [S2O32-]‚ [H+]‚ and water used. [S2O32-]init= __(M[S2O32-])(V[S2O32-])__ [H+]init= _____(M[H+])(V[H+])____ V[S2O32-]+V[H+]+V[water] V[H+]+V[S2O32-]+V[water] Run 1 [S2O32-]init= (0.15 M)(10 mL) (10+3+2)mL = 0.1 M [H+]init= (3 M)(2
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Joshua Annan Biology Section-04 Diffusion and Osmosis December 14‚2012 Alexis‚ Adiba‚ Elliot and Joey Purpose: The purpose of this lab is to get a better understanding of osmosis and diffusion in molecules. We will do this by studying different solutions and how they move through the permeable membrane. Hypothesis: The three different possible predictions were the lab were that the mass would stay the same‚ the mass will increase‚ or the mass will decrease
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Biology Lab T 10:50 The Effects of Alcohol on the Cell Membrane Introduction To understand the effects of alcohol on cell membranes can help one understand more about the effects of alcohol on human cells that are contained in the digestive system and the blood stream. It is important to study this effect due to the fact that it can lead to the understanding of the dangers of alcohol‚ over consumption‚ and even possibly alcohol poisoning. Performing this lab enables the students to have a firsthand
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Lab design –Voltaic Cells Aim Of Experiment In this experiment‚ we are going to make a battery by making an electron current between the metals Zinc and Copper. We aim two study the voltaic cells and the transfer of electrons from the metal to the ion. The tendency for the electrons to flow from one chemical to another‚ such as from the zinc metal to the copper ion as shown here‚ is something that can be channeled and controlled. Channeling the flow of electrons is what we will take up in this
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Instructor: Dr. Bethany Bowling Osmosis and Diffusion Report Estimating the Osmolarity of Plant Cells--Potato YAO ZHANG 03/26/2012 Introduction: It is undeniable that all cells have the kinetic energy. It will led the cells move randomly around to others. For this molecular movement‚ there are two results that might happen. Diffusion is one of them. Diffusion is the movement of molecules that between the high concentration and the low concentration‚ during the molecules move
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Investigating the effect of surface area to volume ratio on Osmosis As far as living organisms are concerned‚ they are all made up of cells whereas‚ the membrane surrounds all those cells. The cell membrane has the key responsibility to maintain a stable interval environment. Even though‚ Cell membrane is made up of phospholipids bilayer and has that great amount flexibility making it unbreakable while transportation of substances. However‚ certain substances such as‚ dissolved gases‚ sugars‚ salt and liquid
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