pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities‚ one of the enzyme that was used in the process was amylase. When a serial dilution was
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Basic Biochemistry CP2085 Effect of Temperature‚ Study and Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature
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generation of secondary messengers that go on to regulate other physiological processes‚ as well as being responsible for the activation of some ion channels and enzymes and assisting in trafficking of proteins to the cell membrane (5‚ 8). Why is PIP2 then so important in hippocampal cells? PIP2 is a substrate for hydrolysis by the enzyme phospholipase C (PLC)‚ whereby the products of this interaction are the secondary messengers‚ diacyl Glycerol (DAG) and inositol trisphosphate (IP3) (6‚ 8) that
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p-nitrophenyl phosphate (a synthetic substrate) at an optimum pH of 10.0 with the liberation of p-nitrophenol. The substrate is colourless‚ but the product p-nitrophenol is yellow in alkaline solution‚ absorbing maximally at 405 nrn. Thus a convenient assay for this enzyme involves monitoring the change in absorbance of the reaction medium at 405 nm. Exergonic (i.e energy producing reactions) exhibit a negative free energy change. Sometimes these reactions occur spontaneously‚ but generally
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An investigation into the effects of temperature on enzyme action An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions. They play a vital role in many aspects of human physiology and are necessary for the functioning of a number of systems‚ for example in the digestive system to help to break down food. All enzymes have a unique active site that can fit on to a particular molecular arrangement on a target substrate; a substance e.g. carbohydrate
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Enzyme kinetics (catalase/kmno4) Enzyme catalysis Farah Mohamed galal 22-3014 t09 Introduction: E + S → ES → E + P Enzymes are proteins which act as a catalyst in biochemical reactions(affect the rate of achemical reaction). The substrate binds to the active site of the enzyme. Any deformation of the active site will affect the activity of the enzyme‚ these are some ways that enzyme action may be affected because of them: 1- Salt concentration: If it is close to zero or very high
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EFFECT OF BIODEGRADABLE SM PLASTIC BAG IN DIFFERENT SUBSTRATE ABSTRACT The purpose of this Investigatory project is to determine the effect of different substrates on the biodegradability of SM plastic bags. The materials needed are: 6‚ 1-litre glass jars; 6‚ 7”x9” aluminums trays; 12 pcs. 3”x4” SM plastic bags; 500g loam soil; 500g compost soil; 500mL seawater; 500mL tap water; Analytical balance; Graduated cylinder; and Weighing scale. The procedure involved cutting the SM plastics bags
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experiment to investigate how the concentration of an acid affects the rate of the reaction. I have set up the experiment’s equipment as shown on figure 1. As you can see by figure 1‚ I have used the reaction between sodium thisoulphate solution and hydrochloric acid (HCL) to come up with results that will help me draw conclusions regarding the effects of the concentration of the acid. I decided to use this particular reaction due to the fact that the product of the reaction between sodium thisoulphate
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investigating how the rate of reaction differs when we change the concentration of Hydrochloric Acid whilst reacting with Magnesium. The rate of reaction is explained by the Collision Theory. This theory explains how various factors affect the reaction rates and how chemical reactions occur. The 4 factors of the Collision Theory are: • Temperature • Concentration • Surface area • Catalyst I am investigating how different concentrations of acids affect the rate of reaction. However I must control
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Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of substrate (sucrose)
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