The effect on rate of an enzyme catalysed reaction by different objectives which include effect of substrate‚ temperature‚ ph and effect of a competitive inhibitor phosphate ions. This is determined by the reaction of hydrolysis by p-nitrophenylphosphate (PNP) as a substrate by the enzyme phosphatase. Abstract The hydrolysis of p-nitrophenyl phosphate has been studied in human red blood cells. To see if hydrolysis was related to the functioning of the sodium pump. Acid phosphatase catalysis’s
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Abstract Enzyme catalysis was observed in order to analyze how changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration affected an enzyme-catalyzed reaction. This experiment analyzed the rate of enzyme-catalyzed reactions and observed the correlation between catalase activity and products formed. It was found out that the rate of an enzyme-catalyzed reaction starts off rapidly‚ decreases‚ and levels off or completely stops‚ and can be further affected by environmental factors
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__________________________________ AP Biology LAB 2. ENZYME CATALYSIS ESSAY 2000 The effects of pH and temperature were studied for an enzyme-catalyzed reaction. The following results were obtained. a. How do (1) temperature and (2) pH affect the activity of this enzyme? In your answer‚ include a discussion of the relationship between the structure and the function of this enzyme‚ as well as a discussion of how structure and function of enzymes are affected by temperature and pH. b. Describe
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lab was to observe and understand the effects of changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions. Hypothesis: I believe that if there is an increase in enzyme concentration‚ an increase in temperature‚ or an increase in pH‚ then the intensity of the reaction will increase‚ which will cause greater
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Chou‚ P. (2007). The effects of background music on the reading performance of taiwanese esl students. New York: Self Published. Retrieved from http://books.google.com/books?id=zVz_Lv9OnN0C&pg=PA33&lpg=PA33&dq=effects of background music on concentration&source=bl&ots=xRZ4pvia4r&sig=lzJhKQmZfw7WXV-WD1hyJhT9I4A&hl=en&sa=X&ei=I3otUeKHBo_o8gSBooHYAw&ved=0CH8Q6AEwCQ * Hallman‚ Price‚ Katsarou‚ S. J. G. (2002‚ November 2).The effects of background music on
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The purpose of this experiment is to measure the effects of changes in temperatures and pH on enzyme activity in skeletal muscle‚ particularly the activity of lactate dehydrogenase (LDH). LDH is a glycolytic enzyme which converts pyruvate to lactate in the following equation: LDH Pyruvate+ NADH ------------ Lactate + NAD The reaction above can move in both directions‚ forward (favored
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different sucrose concentrations then the tube with the lowest concentration of sucrose will expand the potato the most because the water will move into the potato to even out the concentration levels of the sucrose/ water ratio inside and outside of the potato was supported by the data. The data shows that the potato submerged in the lowest concentration of the sucrose water solution had the greatest percent change in mass at 15.2% for 0% concentration. Overall‚ the higher the concentration of the solution
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I. Research Question What are the effects of different sucrose concentrations on potato stripes which have been submerged in a range of solute sucrose concentrations. II. Background Information Osmosis is one of the four methods of moving particles across membranes along with simple diffusion‚ facilitated diffusion‚ and active transport. Water is able to move in and out of most cells freely. Sometimes the number of water molecules moving in and out is the same and there is no net movement‚ but at
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Enzyme Catalysis Lab Problem: Before the lab‚ one should understand: • The general functions and activities of enzymes; • The relationship between the structure and function of enzymes • The concept of initial reaction rates of enzymes; • How the concept of free energy relates to enzyme activity; • That change in temperature‚ pH‚ enzyme concentration‚ and substrate concentration can affect the initial reaction rates of enzyme-catalyzed reactions; and • Catalyst‚ catalysis‚ and catalase
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Enzymes are biological molecules (proteins) that act as catalysts and help complex reactions occur everywhere in life. Enzymes are catalyst for biochemical reactions; Enzymes lower the activation ATP so a reaction can begin over the energy barriers. In this lab‚ a discussion of the enzymes reaction to heat will be addressed. Does heat speed up the enzyme reaction? The prediction is as more heat is applied more reactions will occur then at some point the heat will denature the enzyme as it reaches
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