Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab‚ click on the Experiment button to begin the first assignment. This assignment is designed to help you become
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REPORT Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of substrate (sucrose)
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pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities‚ one of the enzyme that was used in the process was amylase. When a serial dilution was
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1.7 Factors that affect the activity of an enzyme It is important when working with enzymes to understand basic enzymatic theory behind them when selecting conditions to measure the activity of the enzymes. The factors that are known to affect the concentration of enzymes are temperature‚ pH‚ concentration of enzyme‚ concentration of substrate‚ buffer type and concentration‚ the presence of any inhibitors and cofactors (Worthington-biochem.com). 1.7.1. Temperature With most catalysed reactions‚
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affecting Enzyme Activity: The effect of pH on enzyme activity Background Knowledge: An enzyme is a biological catalyst – which speeds up the reaction rate‚ without itself getting altered. Enzymes are proteins with long polypeptide chains that are folded up into three – dimensional shapes. An enzyme acts on a substrate to convert the substrate into a product useful for the organism.The active site is a special region on the surface of the enzyme where the substrate binds to the enzyme.
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Aim To investigate the effect of different temperatures on the activity of rennin in milk Introduction Enzymes are globular protein‚ responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon‚ hydrogen‚ oxygen and nitrogen (Gunsch‚ 2012). The role of enzyme is to act as catalysts‚ substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done
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Background Information Part 1 In the first part of the enzyme lab‚ we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together‚ we observed and saw a change in the color of the substance. The darker and more brown the substance got‚ the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased
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EFFECT OF BIODEGRADABLE SM PLASTIC BAG IN DIFFERENT SUBSTRATE ABSTRACT The purpose of this Investigatory project is to determine the effect of different substrates on the biodegradability of SM plastic bags. The materials needed are: 6‚ 1-litre glass jars; 6‚ 7”x9” aluminums trays; 12 pcs. 3”x4” SM plastic bags; 500g loam soil; 500g compost soil; 500mL seawater; 500mL tap water; Analytical balance; Graduated cylinder; and Weighing scale. The procedure involved cutting the SM plastics bags
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Enzymes are a major role in the process of accelerating chemical reactions. Environmental factors affect the enzymes‚ These environmental factors include ph levels and body temperature. While both of these environmental factors affect the enzyme‚ body temperature affects the enzymes the most and can result in death. Enzymes are affected by high body temperature. Enzymes are highly specific and they work at certain temperature levels depending if it’s hot or cold. However‚ when the
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The Effect of Enzymes on Apple Juice Production Biology Period 6 Background Info The purpose of this experiment was to determine which enzyme or the combination of the two enzymes makes the most apple juice from applesauce. We did his by‚ mixing 10 drops of an enzyme (cellulose/pectinase/both) into a few tablespoons of applesauce. We then left the rest to nature‚ and watched the liquid funnel out for 10 minutes. Lastly‚ we recorded our data. The cell wall is a complicated
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